SMAD6 Antibody (YA5062)
(Synonyms: SMAD6; MADH6; Mothers against decapentaplegic homolog 6; MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6)SMAD6 Antibody (YA5062) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SMAD6.
-
Host:
Mouse
-
Isotype:
IgG1
-
Application:
WB
-
Reactivity :
Human
-
Formulation:
Supplied in purified antibody in PBS with 0.05% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
SMAD6 Antibody (YA5062) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SMAD6.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 53 kDa
Purified recombinant fragment of human SMAD6 expressed in E. Coli. The exact sequence is proprietary to MCE.
Endogenous
Ascitic Fluid
Non-conjugated
Unmodified
IgG1
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in purified antibody in PBS with 0.05% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
SMAD6 is a Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta. Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of pro-inflammatory genes. Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions[1][2][3][4][5][6][7][8][9].
-
Subcellular Localization
Nucleus
-
Expression
Tissue_specificity:It is expressed in the brain, heart, ovaries, peripheral blood leukocytes, small intestine, spleen, thymus, bone marrow, fetal liver, and lymph nodes. -
Isoforms & Post-Translational Modification
O43541 has 3 isomers: O43541-1: 53497 Da (predicted); O43541-2: 26236 Da (predicted); O43541-4: 35467 Da (predicted).
Phosphorylated by BMP type 1 receptor kinase and by PRKX;Monoubiquitinated at Lys-173 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation (PubMed:23455153). Ubiquitinated by WWP1 (By similarity). Ubiquitinated by ARK2C, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling (By similarity);Arginine methylation by PRMT1, which is recruited by BMPR2, initiates BMP-Induced signaling and induces dissociation from the BMPR1B receptor at the cell surface leading to derepress downstream Smad1/Smad5 signaling -
Subunit
Interacts with NEDD4L (By similarity). Interacts with WWP1 (By similarity).
-
SwissProt ID
-
Synonyms
SMAD6; MADH6; Mothers against decapentaplegic homolog 6; MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6
Documentation
Powered by Bioz
