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CHRNA5 Antibody (YA9776)

(Synonyms: CHRNA5, Cholinergic Receptor Nicotinic Alpha 5 Subunit, Acetylcholine Receptor, Nicotinic, Alpha 5 (Neuronal), Cholinergic Receptor, Nicotinic, Alpha 5 (Neuronal), Neuronal Acetylcholine Receptor Subunit Alpha-5, Cholinergic Receptor, Nicotinic Alpha 5, Cholinergic Receptor, Neuronal Nicotinic, Alpha Polypeptide-5, Neuronal Nicotinic Acetylcholine Receptor, Alpha5 Subunit, Cholinergic Receptor, Nicotinic, Alpha Polypeptide 5, NACHRA5, LNCR2)
Customer Review

Based on 1 Customer Validation

CHRNA5 Antibody (YA9776) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to CHRNA5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC, ICC/IF

  • Reactivity :

    Human, mouse, rat

  • Formulation:

    Supplied in PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CHRNA5 Antibody (YA9776)
  • CHRNA5 Antibody (YA9776) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-5000 1:200-2000 1:100-1000

Product Details

Description

CHRNA5 Antibody (YA9776) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to CHRNA5.

  • Host Rabbit
  • Species Reactivity
    Human, mouse, rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Immunogen

A synthesized peptide derived from human CHRNA5

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CHRNA5 Antibody (YA9776)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3) and MCF-7 (lane 4) using CHRNA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    CHRNA5 is a Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. Has an accessory rather than functional role and is only able to form functional nAChRs when co-assembled with another beta subunit. Participates in pentameric assemblies along with CHRNA3, CHRNA4, CHRNB2 and CHRNB4. Increases receptor sensitivity to acetylcholine and nicotine when associated with CHRNA4 and CHRNB2. Plays a role in nicotine addiction[1][2].

  • Subcellular Localization

    Synaptic cell membrane; Cell membrane

  • Isoforms & Post-Translational Modification

    CHRNA5 has an amino acid length of 468, molecular weight is 53054 Da.

  • Subunit

    Neuronal AChR that forms heteropentamers composed of two different type of subunits: alpha and non-alpha (beta).

  • SwissProt ID

    P30532

  • Synonyms

    CHRNA5, Cholinergic Receptor Nicotinic Alpha 5 Subunit, Acetylcholine Receptor, Nicotinic, Alpha 5 (Neuronal), Cholinergic Receptor, Nicotinic, Alpha 5 (Neuronal), Neuronal Acetylcholine Receptor Subunit Alpha-5, Cholinergic Receptor, Nicotinic Alpha 5, Cholinergic Receptor, Neuronal Nicotinic, Alpha Polypeptide-5, Neuronal Nicotinic Acetylcholine Receptor, Alpha5 Subunit, Cholinergic Receptor, Nicotinic, Alpha Polypeptide 5, NACHRA5, LNCR2

References

[1]. Kuryatov A, et al. Acetylcholine receptor (AChR) α5 subunit variant associated with risk for nicotine dependence and lung cancer reduces (α4β2)₂α5 AChR function. Mol Pharmacol. 2011 Jan;79(1):119-25. [Content Brief]

[2]. Wang F, et al. Assembly of human neuronal nicotinic receptor alpha5 subunits with alpha3, beta2, and beta4 subunits. J Biol Chem. 1996 Jul 26;271(30):17656-65. [Content Brief]

CHRNA5 Antibody (YA9776) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3) and MCF-7 (lane 4) using CHRNA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CHRNA5 Antibody (YA9776) Product Image
    1/16
  • Experimental Validation Results for CHRNA5 Antibody (YA9776)
  • CHRNA5 Antibody (YA9776) Product Image
  • Experimental Validation Results for CHRNA5 Antibody (YA9776)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3) and MCF-7 (lane 4) using CHRNA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CHRNA5 Antibody (YA9776)
  • CHRNA5 Antibody (YA9776) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3) and MCF-7 (lane 4) using CHRNA5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CHRNA5 Antibody (YA9776) Product Image WB