MAD1L1 Antibody (YA9774)
(Synonyms: MAD1L1, Mitotic Arrest Deficient 1 Like 1, TXBP181, MAD1, HsMAD1, TP53I9, PIG9, Mitotic Spindle Assembly Checkpoint Protein MAD1, Mitotic Arrest Deficient 1-Like Protein 1, Mitotic Checkpoint MAD1 Protein Homolog, MAD1 Mitotic Arrest Deficient Like 1, Tax-Binding Protein 181, MAD1-Like Protein 1, MAD1 (Mitotic Arrest Deficient, Yeast, Homolog)-Like 1, Mitotic-Arrest Deficient 1, Yeast, Homolog-Like 1, MAD1 Mitotic Arrest Deficient-Like 1 (Yeast), Tumor Protein P53 Inducible Protein 9, HMAD1, MVA7)MAD1L1 Antibody (YA9774) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to MAD1L1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC, ICC/IF
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Reactivity :
Human, mouse, rat
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Formulation:
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:200-2000 | 1:100-1000 |
Product Details
MAD1L1 Antibody (YA9774) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to MAD1L1.
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Host Rabbit
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Species ReactivityHuman, mouse, rat
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 83 kDa,
A synthesized peptide derived from human MAD1
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MAD1L1 is a Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling[1][2][3].
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Subcellular Localization
Nucleus; Chromosome, centromere, kinetochore; Nucleus envelope; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole
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Expression
Tissue_Specificity: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).
Induction: Increased by p53/TP53. -
Isoforms & Post-Translational Modification
MAD1L1 has 3 isoforms, Q9Y6D9-1: amino acid length is 718, molecular weight is 83067 Da (predicted); Q9Y6D9-3: amino acid length is 626, molecular weight is 72286 Da (predicted); Q9Y6D9-4: amino acid length is 671, molecular weight is 77460 Da (predicted).Phosphorylated; by BUB1 (PubMed:10198256). Become hyperphosphorylated in late S through M phases or after mitotic spindle damage (PubMed:9546394). Phosphorylated; by TTK (PubMed:29162720)
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
MAD1L1, Mitotic Arrest Deficient 1 Like 1, TXBP181, MAD1, HsMAD1, TP53I9, PIG9, Mitotic Spindle Assembly Checkpoint Protein MAD1, Mitotic Arrest Deficient 1-Like Protein 1, Mitotic Checkpoint MAD1 Protein Homolog, MAD1 Mitotic Arrest Deficient Like 1, Tax-Binding Protein 181, MAD1-Like Protein 1, MAD1 (Mitotic Arrest Deficient, Yeast, Homolog)-Like 1, Mitotic-Arrest Deficient 1, Yeast, Homolog-Like 1, MAD1 Mitotic Arrest Deficient-Like 1 (Yeast), Tumor Protein P53 Inducible Protein 9, HMAD1, MVA7
Documentation
References
[1]. Jin DY, et al. Mitotic checkpoint locus MAD1L1 maps to human chromosome 7p22 and mouse chromosome 5. Genomics. 1999 Feb 1;55(3):363-4. [Content Brief]
[2]. Nakano H, et al. Nucleoporin translocated promoter region (Tpr) associates with dynein complex, preventing chromosome lagging formation during mitosis. J Biol Chem. 2010 Apr 2;285(14):10841-9. [Content Brief]
[3]. Ji W, et al. Direct interactions of mitotic arrest deficient 1 (MAD1) domains with each other and MAD2 conformers are required for mitotic checkpoint signaling. J Biol Chem. 2018 Jan 12;293(2):484-496. [Content Brief]
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