LAMP2 Antibody (YA6257)

Art. -Nr.: HY-P86565: Data Sheet COA
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User Guide for Antibodies
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LAMP2 Antibody (YA6257) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LAMP2.

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50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

LAMP2 Antibody (YA6257) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LAMP2.

Host

Rabbit

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 45 kDa;

Observed band size: 120 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P13473

Gene ID

3920 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000
IP IP: Immunoprecipitation	1:50-1:200

Reinheit	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032, PubMed:36586411, PubMed:37390818, PubMed:8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032, PubMed:36586411, PubMed:8662539). Functions by binding target proteins, such as GAPDH, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:36586411, PubMed:8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed:36586411). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:27628032). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (PubMed:15894275, PubMed:20518820). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (PubMed:20518820); Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII; (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F

Subcellular Localization：Lysosome membrane; Single-pass type I membrane protein; Endosome membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Cytoplasmic vesicle, autophagosome membrane

Expression：
Tissue_specificity:LAMP-2A isoform is highly expressed in the placenta, lungs, and liver; lower expression in the kidneys and pancreas; and extremely low expression in the brain and skeletal muscle (PubMed:26856698, PubMed:7488019) . LAMP-2B isoform is expressed in the spleen, thymus, prostate, testes, small intestine, colon, skeletal muscle, brain, placenta, lungs, kidneys, ovaries, pancreas, and liver (PubMed:26856698, PubMed:7488019) . LAMP-2C is expressed in the small intestine, colon, heart, brain, and skeletal muscle; and lower expression in the kidneys and placenta (PubMed:26856698) .

Induction:In peripheral blood B cells isoform LAMP-2A, LAMP-2B and LAMP-2C are up-regulated in response to treatments that stimulate immune responses via the Toll-like receptors TLR7 or TLR9

Isoforms & Post-Translational Modification：P13473 has 3 isomers: P13473-1: 44961 Da (predicted); P13473-2: 44956 Da (predicted); P13473-3: 45170 Da (predicted).
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans

Subunit：Monomer (PubMed:18644871, PubMed:25342746). Homodimer (PubMed:25342746). Homotrimer (PubMed:25342746). Forms large homooligomers (PubMed:18644871). Interacts (via its cytoplasmic region) with HSPA8; HSPA8 mediates recruitment of proteins with a KFERQ motif to the surface of the lysosome for chaperone-mediated autophagy (PubMed:25342746, PubMed:36586411). Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity). Interacts with MLLT11 (PubMed:24880125). Interacts with ABCB9 (PubMed:22641697). Interacts with FURIN (PubMed:32295904). Interacts with CT55; this interaction may be important for LAMP2 protein stability (PubMed:36481789). Interacts with TMEM175; inhibiting the proton channel activity of TMEM175 (PubMed:37390818). Forms a ternary complex with RAB7A and RUFY4 (via RUN domain); the interaction with RAB7A is mediated by RUFY4 (via RUN and coiled coil domains) (By similarity)

Synonyms

LAMP2; Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; CD107b

Dokumentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)