1. Antibodies
  2. Primary Antibodies
  3. Lactate Dehydrogenase Antibody

Lactate Dehydrogenase Antibody

Cat. No.: HY-P80422
COA User Guide for Antibodies

Lactate Dehydrogenase Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 37 kDa, targeting to Lactate Dehydrogenase. It can be used for WB,ICC/IF,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Lactate Dehydrogenase Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 37 kDa, targeting to Lactate Dehydrogenase. It can be used for WB,ICC/IF,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse.

Tag

Free

Molecular Weight

Predicted band size: 37 kDa

Purity

Protein A affinity purified.

Subcellular Location

Cytoplasm.

Conjugation

Non-conjugated

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse

SwissProt ID

SwissProt: P07195 Human;SwissProt: P00338 Human;SwissProt: P16125 Mouse;SwissProt: P06151 Mouse;SwissProt: P42123 Rat;SwissProt: P04642 Rat

Dilution Ratio

WB: 1:1,000-1:5,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:200-1:2,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

  • Western blot analysis of extracts from Hela(lane 2(10μg) or (lane 3(20μg)) and HEK-293(lane 4(10μg) or (lane 5(20μg)), using Lactate Dehydrogenase (HY-P80422) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80422, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB, ICC/IF, IHC-P, FC, IP

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Immunogen

Synthetic peptide corresponding to Human LDHB.AA range:270-310.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Lactate Dehydrogenase Antibody
Cat. No.:
HY-P80422
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