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β-actin Antibody (YA7506)

(Synonyms: Beta-actin, ACTB)
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Based on 1 publication(s) in Google Scholar

β-actin Antibody (YA7506) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to β-actin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for β-actin Antibody (YA7506)
  • β-actin Antibody (YA7506) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:2000-1:20000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

β-actin Antibody (YA7506) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to β-actin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for β-actin Antibody (YA7506)
    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), Caco-2 (lane 4), MCF-7 (lane 5), and HeLa (lane 6) using β-actin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Actin exists in three major isoforms, including α-, β-, and γ-actin. β-Aactin is primarily found in nonmuscle cells (e.g., β-cytoplasmic) . β-Actin is a highly conserved cytoskeletal protein found in nearly all eukaryotic cells. It is an essential structural component of the cytoskeleton, playing a crucial role in cell motility, division, structural integrity, and intracellular signaling. β-Actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples.

  • Subcellular Localization

    Cytoplasm, cytoskeleton,Nucleus

  • Isoforms & Post-Translational Modification

    P60709: 375 amino acids, molecular weight 41737 Da.

  • Subunit

    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix (PubMed:16685646, PubMed:28604741)

  • SwissProt ID

    P60709

  • Synonyms

    Beta-actin, ACTB

β-actin Antibody (YA7506) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), Caco-2 (lane 4), MCF-7 (lane 5), and HeLa (lane 6) using β-actin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • β-actin Antibody (YA7506) Product Image
    1/16
  • Experimental Validation Results for β-actin Antibody (YA7506)
  • β-actin Antibody (YA7506) Product Image
  • Experimental Validation Results for β-actin Antibody (YA7506)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), Caco-2 (lane 4), MCF-7 (lane 5), and HeLa (lane 6) using β-actin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for β-actin Antibody (YA7506)
  • β-actin Antibody (YA7506) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), A549 (lane 3), Caco-2 (lane 4), MCF-7 (lane 5), and HeLa (lane 6) using β-actin antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • β-actin Antibody (YA7506) Product Image WB