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HDAC7 Antibody (YA7730)

(Synonyms: HDAC7A, HDAC7, Histone deacetylase 7, HD7, Histone deacetylase 7A, Protein deacetylase HDAC7, HD7a)
Customer Review

Based on 1 Customer Validation

HDAC7 Antibody (YA7730) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue, FC, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • HDAC7 Antibody (YA7730) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:100-500 1:50-100 1:50-200

Product Details

Description

HDAC7 Antibody (YA7730) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC7.

  • Host Rabbit
  • Clonality Recombinant
  • Species Reactivity
    Human Predicted Reactivity: Human,Mouse,Rat
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Calculated Molecular Weight Predicted band size: 105 kDa;
Immunogen

A synthesized peptide derived from human HDAC7: 35-200

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human liver cacner using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human placenta using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human heart using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human melanoma using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    Immunohistochemical analysis of paraffin-embedded human spleen using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    HDAC7 (histone deacetylase 7) is a class IIa histone deacetylase that functions as a signal-responsive transcriptional coregulator linking extracellular stimuli to chromatin-dependent gene regulation and cell fate control[1][2]. HDAC7 undergoes regulated nucleo-cytoplasmic shuttling through phosphorylation-dependent mechanisms, allowing it to integrate signaling pathways with transcriptional programs that govern development, immunity, and vascular biology[1][3]. Mechanistically, HDAC7 participates in the control of endothelial growth, migration, angiogenesis, and vascular integrity, and VEGF-dependent signaling can promote HDAC7 phosphorylation and redistribution to regulate endothelial responses[3][4][5]. In disease-associated contexts, aberrant HDAC7 expression has been linked to tumor progression, metastasis, vascular microenvironment remodeling, and therapeutic resistance, supporting its relevance as a candidate biomarker and experimental target in cancer research[6]. Compared with related class IIa isoforms, including HDAC4, HDAC5, and HDAC9, HDAC7 is distinguished by its prominent role as a signaling hub that couples cellular localization dynamics with transcriptional regulation in endothelial and immune systems[1][2]. Furthermore, class IIa HDACs possess relatively low intrinsic deacetylase activity and frequently function through protein-protein interactions and corepressor complexes; HDAC7 has been reported to associate with HDAC3, highlighting a mechanistic distinction from catalytically active class I HDACs[7]. For experimental applications, HDAC-targeting compounds such as vorinostat affect HDAC7 among other HDAC family members, and ongoing efforts to improve isoform selectivity continue to inform the design of epigenetic and cancer-focused research strategies[6][8].

  • Subcellular Localization

    Nucleus,Cytoplasm

  • Isoforms & Post-Translational Modification

    Q8WUI4 has 10 isomers: Q8WUI4-1: 102927 Da (predicted); Q8WUI4-2: 51829 Da (predicted); Q8WUI4-3: 99093 Da (predicted); Q8WUI4-4: 99755 Da (predicted); Q8WUI4-5: 106740 Da (predicted); Q8WUI4-6: 105111 Da (predicted); Q8WUI4-7: 102906 Da (predicted); Q8WUI4-8: 108937 Da (predicted); Q8WUI4-9: 46041 Da (predicted); Q8WUI4-10: 66187 Da (predicted).
    May be phosphorylated by CaMK1

  • Subunit

    Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3 (PubMed:11466315)

  • SwissProt ID

    Q8WUI4

  • Synonyms

    HDAC7A, HDAC7, Histone deacetylase 7, HD7, Histone deacetylase 7A, Protein deacetylase HDAC7, HD7a

References

[1]. Atochin DN, et al. The phosphorylation state of eNOS modulates vascular reactivity and outcome of cerebral ischemia in vivo. J Clin Invest. 2007 Jul;117(7):1961-7. [Content Brief]

[2]. Margariti A, et al. Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin. Circ Res. 2010 Apr 16;106(7):1202-11. [Content Brief]

[3]. Yin X, et al. Multiple red blood cell flows through microvascular bifurcations: cell free layer, cell trajectory, and hematocrit separation. Microvasc Res. 2013 Sep;89:47-56. [Content Brief]

[4]. Liu C, et al. HDAC7: a promising target in cancer. Front Oncol. 2024 Feb 28;14:1327933. [Content Brief]

[5]. Rabezanahary H, et al. Live virus neutralizing antibodies against pre and post Omicron strains in food and retail workers in Québec, Canada. Heliyon. 2024 May 21;10(10):e31026. [Content Brief]

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HDAC7 Antibody (YA7730) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cacner using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human melanoma using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    1/16
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • HDAC7 Antibody (YA7730) Product Image
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cacner using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human heart using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human melanoma using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • Experimental Validation Results for HDAC7 Antibody (YA7730)
  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cacner using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human heart using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human melanoma using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen using HDAC7 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P88045, 1/500) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HDAC7 Antibody (YA7730) Product Image IHC-P
  • HDAC7 Antibody (YA7730) Product Image IHC-P
  • HDAC7 Antibody (YA7730) Product Image IHC-P
  • HDAC7 Antibody (YA7730) Product Image IHC-P
  • HDAC7 Antibody (YA7730) Product Image IHC-P
  • HDAC7 Antibody (YA7730) Product Image IHC-P
  • HDAC7 Antibody (YA7730) Product Image IHC-P