JAM3 Antibody (YA4464)
(Synonyms: JAMC; JAM-2; JAM-3; JAM-C)JAM3 Antibody (YA4464) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to JAM3.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
JAM3 Antibody (YA4464) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to JAM3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Purified recombinant fragment of human JAM3 (AA: extra 32-241) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Junctional adhesion molecule 3 (JAM3) functions as a critical mediator of cell-cell adhesion within endothelial and epithelial tissues[1]. Mechanistically, JAM3 engages in heterotypic interactions with JAM2, facilitating integrin-dependent adhesion processes, particularly via α4β1 integrins in T cells[2]. This JAM3-JAM2 interaction is cation-dependent and can be modulated by integrin inhibitors, highlighting its therapeutic potential in immune modulation[2]. Compared with related isoforms such as JAM1 and JAM-A, JAM3 displays unique binding specificity and localization patterns, enabling distinct contributions to transendothelial migration and barrier formation[1][3]. In disease models, JAM3 deficiency has been linked to impaired leukocyte recruitment and altered vascular permeability, emphasizing its relevance in inflammatory and vascular pathologies[1]. Experimental applications exploit JAM3 engagement to study T cell adhesion dynamics, while selective inhibition of JAM3-JAM2 interactions allows controlled modulation of immune cell trafficking[2]. Overall, JAM3 integrates adhesion signaling with cellular polarity and immune responses, distinguishing it from other JAM family members both functionally and spatially[3].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell junction; Cell junction, desmosome; Cell junction, tight junction; Secreted
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Expression
Tissue_specificity:Detected on round and elongated spermatids (at protein level) (PubMed:15372036) . Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level) . Also detected in the synovial fluid of with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level) -
Isoforms & Post-Translational Modification
Q9BX67 has 2 isomers: Q9BX67-1: 35020 Da (predicted); Q9BX67-2: 29223 Da (predicted).
Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by pro-inflammatory factors;S-palmitoylated by ZDHHC7. S-palmitoylation promotes expression at tight junctions -
Subunit
Interacts with ITGAM (PubMed:12208882, PubMed:15194813).
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SwissProt ID
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Synonyms
JAMC; JAM-2; JAM-3; JAM-C
Documentation
[1]. Cunningham SA, et al. JAM2 interacts with alpha4beta1. Facilitation by JAM3. J Biol Chem. 2002 Aug 2;277(31):27589-92. [Content Brief]
[2]. Ebnet K. JAM-A and aPKC: A close pair during cell-cell contact maturation and tight junction formation in epithelial cells. Tissue Barriers. 2013 Jan 1;1(1):e22993. doi: 10.4161/tisb.22993. PMID: 24665372; PMCID: PMC3879182. et al. JAM-A and aPKC: A close pair during cell-cell contact maturation and tight junction formation in epithelial cells. Tissue Barriers. 2013 Jan 1;1(1):e22993. [Content Brief]
[3]. Ostermann G, et al. JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes. Nat Immunol. 2002 Feb;3(2):151-8. [Content Brief]
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