JAM3 Antibody (YA4464)

Cat. No.: HY-P84767: Data Sheet User Guide for Antibodies
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JAM3 Antibody (YA4464) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to JAM3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

JAM3 Antibody (YA4464) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to JAM3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BX67

Gene ID

83700 [NCBI]

Immunogen

Purified recombinant fragment of human JAM3 (AA: extra 32-241) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes (PubMed:11590146, PubMed:11823489). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed:11590146, PubMed:24357068). Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium (By similarity). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed:12208882, PubMed:15194813). Plays a role in angiogenesis (PubMed:23255084). Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion (By similarity); Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell junction; Cell junction, desmosome; Cell junction, tight junction; Secreted

Expression：
Tissue_specificity:Detected on round and elongated spermatids (at protein level) (PubMed:15372036) . Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level) . Also detected in the synovial fluid of with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level)

Isoforms & Post-Translational Modification：Q9BX67 has 2 isomers: Q9BX67-1: 35020 Da (predicted); Q9BX67-2: 29223 Da (predicted).
Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by pro-inflammatory factors;S-palmitoylated by ZDHHC7. S-palmitoylation promotes expression at tight junctions

Subunit：Interacts with ITGAM (PubMed:12208882, PubMed:15194813).

Synonyms

JAMC; JAM-2; JAM-3; JAM-C

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hcc1937-cell.html [Content Brief]