ATG5 Antibody (YA4487)

製品番号: HY-P84790: データシート User Guide for Antibodies
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ATG5 Antibody (YA4487) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ATG5.

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容量	価格（税別）	在庫状況	数量
20 μL	$167	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	$252	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	$408	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

Synthetic products have potential research and development risk.

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* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

ATG5 Antibody (YA4487) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to ATG5.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 32 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9H1Y0

Gene ID

9474 [NCBI]

Immunogen

Purified recombinant fragment of human ATG5 (AA: 144-275) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

純度	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity); May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD; (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed:19666601)

Subcellular Localization：Cytoplasm; Preautophagosomal structure membrane; Peripheral membrane protein

Expression：
Tissue_specificity:It is ubiquitous. The levels of mRNA are similar in living cells and apoptotic cells, while protein expression is significantly elevated in apoptotic cells.

Induction:By apoptotic stimuli

Isoforms & Post-Translational Modification：Q9H1Y0 has 2 isomers: Q9H1Y0-1: 32447 Da (predicted); Q9H1Y0-2: 22931 Da (predicted).
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane;Acetylated by EP300

Subunit：Forms a conjugate with ATG12 (PubMed:11825910, PubMed:12207896, PubMed:17709747, PubMed:26812546). Part of the minor complex composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa); this complex interacts with ATG3 leading to disruption of ATG7 interaction and promotion of ATG8-like proteins lipidation (PubMed:24191030). Forms an 800-kDa complex composed of ATG12-ATG5 and ATG16L2 (By similarity). The ATG12-ATG5 conjugate interacts with RAB33A; this interaction is bridged by ATG16L1 and promotes ATG12-ATG5-ATG16L1 complex recruitment to phagophores (PubMed:32960676). Interacts with TECPR1; the interaction is direct and does not take place when ATG16L1 is associated with the ATG5-ATG12 conjugate (PubMed:21575909, PubMed:22342342). Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate form. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Interacts with ATG3 (PubMed:11825910, PubMed:12207896). Interacts with ATG7 and ATG10 (By similarity). Interacts with FADD (PubMed:15778222). Interacts with Bassoon/BSN; this interaction is important for the regulation of presynaptic autophagy (By similarity). Interacts with ATG16L2 (By similarity)

Synonyms

ASP; APG5; APG5L; hAPG5; APG5-LIKE

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/hct-116-cell.html [Content Brief]