WTAP Antibody (YA1657)
(Synonyms: hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein; Pre-mRNA-splicing regulator WTAP)WTAP Antibody (YA1657) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to WTAP.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, IHC-P, IP
-
Reactivity :
Human
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
IP
IP: Immunoprecipitation
|
|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
WTAP Antibody (YA1657) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to WTAP.
-
Host Rabbit
-
Clonality Recombinant, Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 44 kDa
Recombinant protein of human WTAP
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
WTAP is an Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes[1][2][3][4][5][6][7].
-
Subcellular Localization
Nucleus speckle; Nucleus, nucleoplasm; Cytoplasm
-
Expression
Tissue_specificity:general expression
Induction:In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative -
Isoforms & Post-Translational Modification
Q15007 has 2 isomers: Q15007-1: 44244 Da (predicted); Q15007-2: 17801 Da (predicted).
-
Subunit
Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:24316715, PubMed:24407421, PubMed:24981863, PubMed:29348140, PubMed:29506078, PubMed:29507755). The MAC subcomplex is composed of METTL3 and METTL14 (PubMed:29507755). The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B) (PubMed:29507755). Interacts with WT1 (PubMed:11001926, PubMed:17095724). Also a component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (PubMed:24100041)
-
SwissProt ID
-
Synonyms
hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein; Pre-mRNA-splicing regulator WTAP
-
Research Field
Epigenetics and Nuclear Signaling
Documentation
Powered by Bioz
