CD166 Antibody (YA4252)(PBS only)

Cat. No.: HY-P84555A: Fiche technique User Guide for Antibodies
FAQs
Technical Support

CD166 Antibody (YA4252) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD166.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

* Veuillez sélectionner la quantité avant d'ajouter des articles.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

CD166 Antibody (YA4252) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD166.

Host

Mouse

Masse moléculaire

Predicted band size: 65 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13740

Gene ID

214 [NCBI]

Immunogen

Purified recombinant fragment of human CD166 (AA: extra 227-381) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureté	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:15048703, PubMed:15496415, PubMed:16352806, PubMed:23169771, PubMed:24945728, PubMed:7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed:29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity); Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell projection, axon; Cell projection, dendrite; Secreted

Expression：
Tissue_specificity:Detected on hematopoietic stem cells derived from umbilical cord blood (PubMed:24740813) . Detected on lymph vessel endothelial cells, skin and tonsil (PubMed:23169771) . Detected on peripheral blood monocytes (PubMed:15048703) . Detected on monocyte-derived dendritic cells (at protein level) (PubMed:16352806) . Detected at low levels in spleen, placenta, liver (PubMed:9502422) . Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells (PubMed:7760007) . Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells (PubMed:15496415) . Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines (PubMed:9502422)

Induction:Up-regulated by TNF and IFNG (at protein level)

Isoforms & Post-Translational Modification：Q13740 has 4 isomers: Q13740-1: 65102 Da (predicted); Q13740-2: 63665 Da (predicted); Q13740-3: 15109 Da (predicted); Q13740-4: 33878 Da (predicted).
Glycosylated

Subunit：Homodimer (PubMed:15048703, PubMed:16352806, PubMed:26146185, PubMed:7760007). Interacts (via extracellular domain) with CD6 (via extracellular domain) (PubMed:15048703, PubMed:16914752, PubMed:24945728, PubMed:26146185, PubMed:7760007, PubMed:8823162).

Synonyms

MEMD; ALCAM

Documentation

User Guide for Antibodies (1077 KB)