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EPO Antibody (YA3605)

(Synonyms: EP; MVCD2; MGC138142; EPO)
Customer Review

Based on 1 Customer Validation

EPO Antibody (YA3605) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EPO.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ICC
  • Experimental Validation Results for EPO Antibody (YA3605)
  • Experimental Validation Results for EPO Antibody (YA3605)
  • Experimental Validation Results for EPO Antibody (YA3605)
  • EPO Antibody (YA3605) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

EPO Antibody (YA3605) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EPO.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Immunogen

Purified recombinant fragment of human EPO aa 28-193.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718792

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EPO Antibody (YA3605)
    Immunofluorescence analysis of GC-7901 cells labeling EPO antibody (HY-P83908) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for EPO Antibody (YA3605)
    Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EPO Antibody (YA3605)
    Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    EPO is a Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3[1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Produced by the kidneys or liver of adult mammals, and by the liver of fetuses or newborn mammals.

  • SwissProt ID

    P01588

  • Gene ID
    2056 [NCBI]
  • Synonyms

    EP; MVCD2; MGC138142; EPO

EPO Antibody (YA3605) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of GC-7901 cells labeling EPO antibody (HY-P83908) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EPO Antibody (YA3605) Product Image
    1/16
  • Experimental Validation Results for EPO Antibody (YA3605)
  • Experimental Validation Results for EPO Antibody (YA3605)
  • Experimental Validation Results for EPO Antibody (YA3605)
  • EPO Antibody (YA3605) Product Image
  • Experimental Validation Results for EPO Antibody (YA3605)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of GC-7901 cells labeling EPO antibody (HY-P83908) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for EPO Antibody (YA3605)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EPO Antibody (YA3605)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for EPO Antibody (YA3605)
  • Experimental Validation Results for EPO Antibody (YA3605)
  • Experimental Validation Results for EPO Antibody (YA3605)
  • EPO Antibody (YA3605) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of GC-7901 cells labeling EPO antibody (HY-P83908) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • EPO Antibody (YA3605) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EPO Antibody (YA3605) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling EPO with EPO Antibody (HY-P83908) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with EPO Antibody (HY-P83908) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • EPO Antibody (YA3605) Product Image ICC
  • EPO Antibody (YA3605) Product Image ICC
  • EPO Antibody (YA3605) Product Image ICC