Phospholipase C beta 3/PLCB3 Antibody (YA9824)
(Synonyms: PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3)
Based on 1 Customer Validation
Phospholipase C beta 3/PLCB3 Antibody (YA9824) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospholipase C beta 3/PLCB3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000 | 1:2000 |
Product Details
Phospholipase C beta 3/PLCB3 Antibody (YA9824) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospholipase C beta 3/PLCB3.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 150 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 139 kDa
Synthetic peptide within Human Phospholipase C beta 3/PLCB3 aa 1,161-1,210 / 1,234.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Phospholipase C beta 3/PLCB3 catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Key transducer of G protein-coupled receptor signaling: activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation. In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses (By similarity). In conjunction with OSBPL2, it may contribute to the control of keratinocyte proliferation and differentiation, probably through the regulation of the ERK pathway and the cell cycle[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Membrane; Nucleus
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Isoforms & Post-Translational Modification
Phospholipase C beta 3/PLCB3 has 2 isoforms, Q01970-1: amino acid length is 1234, molecular weight is 138799 Da (predicted); Q01970-2: amino acid length is 1167, molecular weight is 131205 Da (predicted).
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Subunit
Interacts with SHANK2 (By similarity).
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SwissProt ID
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Synonyms
PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3
Documentation
References
[1]. Waldo GL, et al. Kinetic scaffolding mediated by a phospholipase C-beta and Gq signaling complex. Science. 2010 Nov 12;330(6006):974-80. [Content Brief]
[2]. Ben-Salem S, et al. Defect in phosphoinositide signalling through a homozygous variant in PLCB3 causes a new form of spondylometaphyseal dysplasia with corneal dystrophy. J Med Genet. 2018 Feb;55(2):122-130. [Content Brief]
[3]. Falzone ME, et al. Gβγ activates PIP2 hydrolysis by recruiting and orienting PLCβ on the membrane surface. Proc Natl Acad Sci U S A. 2023 May 16;120(20):e2301121120. [Content Brief]
[4]. Falzone ME, et al. The mechanism of Gα(q) regulation of PLCβ3-catalyzed PIP2 hydrolysis. Proc Natl Acad Sci U S A. 2023 Nov 28;120(48):e2315011120. [Content Brief]
[5]. Tall E, et al. Phosphoinositide binding specificity among phospholipase C isozymes as determined by photo-cross-linking to novel substrate and product analogs. Biochemistry. 1997 Jun 10;36(23):7239-48. [Content Brief]
[6]. Wang Y, et al. OSBPL2 compound heterozygous variants cause dyschromatosis, ichthyosis, deafness and atopic disease syndrome. Biochim Biophys Acta Mol Basis Dis. 2024 Jun;1870(5):167207. [Content Brief]
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