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MC4R Antibody (YA1645)

(Synonyms: MC4R; Melanocortin 4 receptor)
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Based on 1 Customer Validation

MC4R Antibody (YA1645) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MC4R.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA or 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol..

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MC4R Antibody (YA1645)
  • MC4R Antibody (YA1645) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MC4R Antibody (YA1645) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MC4R.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MC4 Receptor aa1-50/332.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103997

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA or 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol..

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MC4R Antibody (YA1645)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), JAR (lane 2, 15 μg), and PC-12 (lane 3, 15 μg) using MC4R Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    MC4R (melanocortin-4 receptor) is a class A G protein-coupled receptor that functions as a central regulator of appetite, energy expenditure, and body-weight homeostasis within the hypothalamic leptin-melanocortin network[1][2]. Activation of proopiomelanocortin (POMC) neurons promotes release of α-melanocyte-stimulating hormone (α-MSH), which activates MC4R signaling and suppresses food intake while increasing energy expenditure, establishing MC4R as a key effector of energy balance regulation[3][1]. Mechanistically, MC4R integrates neuroendocrine signals controlling satiety and metabolic homeostasis, and disruption of MC4R signaling impairs these regulatory processes[2][4]. Disease relevance is strongly supported by human genetics, as MC4R deficiency represents the most common monogenic cause of obesity, and loss-of-function variants are associated with early-onset severe obesity, hyperphagia, and metabolic abnormalities[4][5][6]. In experimental and clinical studies, MC4R pathway dysfunction has therefore become a widely used model for investigating genetic obesity and energy-homeostasis disorders[2][4]. Compared with the closely related melanocortin receptor MC3R, MC4R exhibits a distinct and non-redundant role in body-weight regulation; studies in knockout models indicate that MC3R and MC4R contribute independently and complementarily to energy homeostasis rather than serving interchangeable functions[7]. For experimental applications, selective MC4R agonists, particularly setmelanotide, have demonstrated the ability to restore signaling in subsets of impaired MC4R variants and induce weight loss in MC4R-deficient obesity, supporting their utility as pharmacological tools and therapeutic candidates for melanocortin-pathway research[8][9][10].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Brain, placental, and gut tissues

  • Subunit

    Homodimer; disulfide-linked, also forms higher order oligomers. Interacts with GNAS (PubMed:33858992). Interacts with ATRNL1 (By similarity). Interacts with MGRN1; this interaction competes with GNAS-binding and thus inhibits agonist-induced cAMP production. Interacts with MRAP and MRAP2; these associated factors increase ligand-sensitivity and generation of cAMP

  • SwissProt ID

    P32245

  • Gene ID
    4160 [NCBI]
  • Synonyms

    MC4R; Melanocortin 4 receptor

  • Research Field

    Neuroscience

References

[1]. Yeo GSH, et al. The melanocortin pathway and energy homeostasis: From discovery to obesity therapy. Mol Metab. 2021;48:101206.

[2]. Fatima MT, et al. Melanocortin-4 receptor complexity in energy homeostasis,obesity and drug development strategies. Diabetes Obes Metab. 2022 Apr;24(4):583-598. [Content Brief]

[3]. D'Agostino G, et al. Alpha-melanocyte stimulating hormone: production and degradation. J Mol Med (Berl). 2010 Dec;88(12):1195-201. [Content Brief]

[4]. Farooqi IS, et al. Clinical spectrum of obesity and mutations in the melanocortin 4 receptor gene. N Engl J Med. 2003 Mar 20;348(12):1085-95. [Content Brief]

[5]. Sridhar GR, et al. Melanocortin 4 receptor mutation in obesity. World J Exp Med. 2024 Dec 20;14(4):99239. [Content Brief]

[6]. Giannopoulou EZ, et al. Monogenic obesity due to MC4R deficiency: lessons from a multigenerational case. Mol Cell Pediatr. 2026 Jan 5;13(1):3. [Content Brief]

[7]. Micioni Di Bonaventura E, et al. The Melanocortin System behind the Dysfunctional Eating Behaviors. Nutrients. 2020 Nov 14;12(11):3502. [Content Brief]

[8]. Collet TH, et al. Evaluation of a melanocortin-4 receptor (MC4R) agonist (Setmelanotide) in MC4R deficiency. Mol Metab. 2017;6(10):1321-1329.

[9]. Kühnen P, et al. Melanocortin-4 Receptor Signalling: Importance for Weight Regulation and Obesity Treatment. Trends Mol Med. 2019;25(2):136-148.

[10]. Sun Y, et al. The effect of melanocortin-4 receptor agonist drugs on obesity and metabolic risk factors: a systematic review and meta-analysis. Diabetol Metab Syndr. 2025 Dec 28;18(1):40. [Content Brief]

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MC4R Antibody (YA1645) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), JAR (lane 2, 15 μg), and PC-12 (lane 3, 15 μg) using MC4R Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MC4R Antibody (YA1645) Product Image
    1/16
  • Experimental Validation Results for MC4R Antibody (YA1645)
  • MC4R Antibody (YA1645) Product Image
  • Experimental Validation Results for MC4R Antibody (YA1645)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), JAR (lane 2, 15 μg), and PC-12 (lane 3, 15 μg) using MC4R Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for MC4R Antibody (YA1645)
  • MC4R Antibody (YA1645) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), JAR (lane 2, 15 μg), and PC-12 (lane 3, 15 μg) using MC4R Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • MC4R Antibody (YA1645) Product Image WB