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EPCAM Antibody (YA3827)

(Synonyms: ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1)
Customer Review

Based on 1 Customer Validation

EPCAM Antibody (YA3827) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EPCAM.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • EPCAM Antibody (YA3827) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

EPCAM Antibody (YA3827) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EPCAM.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Purified recombinant fragment of human EPCAM (AA: 24-265) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718843

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Western blot analysis of extracts from HT-29 (lane2(20μg), A431(lane3(20μg), HCT-116(lane4(20μg) and MCF-7(lane5(20μg) using EPCAM Antibody (HY-P84130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human Liver cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    EpCAM (CD326) is a transmembrane glycoprotein expressed on the surface of epithelial cells and serves as a classic marker for epithelial cells and epithelial-derived tumors. It plays a role in cell adhesion, the regulation of proliferation, and the maintenance of tumor stemness, holding significant value in tumor diagnosis, circulating tumor cell detection, cancer stem cell research, and the development of targeted therapies.

  • Subcellular Localization

    Lateral cell membrane; Single-pass type I membrane protein; Cell junction, tight junction

  • Expression


    Tissue_specificity:This gene is highly and selectively expressed in undifferentiated embryonic stem cells (ESCs) , but not in differentiated ESCs. Once ESCs differentiate, its protein level rapidly declines. This gene is expressed on almost all epithelial cell membranes, but not on mesoderm or neural cell membranes. It has also been found on the surface of adenocarcinomas.

  • Subunit

    Monomer. Interacts with phosphorylated CLDN7

  • SwissProt ID

    P16422

  • Gene ID
    4072 [NCBI]
  • Synonyms

    ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1

EPCAM Antibody (YA3827) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane2(20μg), A431(lane3(20μg), HCT-116(lane4(20μg) and MCF-7(lane5(20μg) using EPCAM Antibody (HY-P84130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image
    1/16
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • EPCAM Antibody (YA3827) Product Image
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29 (lane2(20μg), A431(lane3(20μg), HCT-116(lane4(20μg) and MCF-7(lane5(20μg) using EPCAM Antibody (HY-P84130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • Experimental Validation Results for EPCAM Antibody (YA3827)
  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29 (lane2(20μg), A431(lane3(20μg), HCT-116(lane4(20μg) and MCF-7(lane5(20μg) using EPCAM Antibody (HY-P84130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cancer‌ using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Endometrial carcinoma using EPCAM antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P84130, 1:400 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue using EPCAM Antibody (HY-P84130, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EPCAM Antibody (YA3827) Product Image WB
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P
  • EPCAM Antibody (YA3827) Product Image IHC-P