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HADH Antibody (YA6588)

(Synonyms: 3 hydroxyacyl Coenzyme A dehydrogenase antibody; HAD antibody; HADH antibody; HADH1 antibody; HADHSC antibody; HADHSC, formerly antibody; HADSC, formerly antibody; HCDH antibody; HCDH_HUMAN antibody; HHF4 antibody; 3 hydroxyacyl Coenzyme A dehydrogenase antibody; HAD antibody; HADH antibody; HADH1 antibody; HADHSC antibody; HADHSC, formerly antibody; HADSC, formerly antibody; HCDH antibody; HCDH_HUMAN antibody; HHF4 antibody; Hydroxyacyl CoA dehydrogenase antibody; Hydroxyacyl-coenzyme A dehydrogenase antibody; hydroxyacyl-coenzyme A dehydrogenase, mitochondrial antibody; L 3 hydroxyacyl Coenzyme A dehydrogenase short chain antibody; M SCHAD antibody; Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase antibody; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase antibody; MGC8392 antibody; mitochondrial antibody; MSCHAD antibody; OTTHUMP00000162626 antibody; OTTHUMP00000219688 antibody; SCHAD antibody; SCHAD, formerly antibody; Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial antibody; short chain 3-hydroxyacyl-coa dehydrogenase antibody; Short-chain 3-hydroxyacyl-CoA dehydrogenase antibody; )
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Based on 1 Customer Validation

HADH Antibody (YA6588) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HADH.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HADH Antibody (YA6588)
  • HADH Antibody (YA6588) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:200 1:200-1:1:1000

Product Details

Description

HADH Antibody (YA6588) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HADH.

  • Host Mouse
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Immunogen

Synthetic peptide within Human HADH aa 265-314 / 314.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HADH Antibody (YA6588)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using HADH Antibody (HY-P86895) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    HADH is a Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion. Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes[1][2][3].

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_Specificity: Expressed in liver, kidney, pancreas, heart and skeletal muscle

  • Isoforms & Post-Translational Modification

    Q16836 has three isomers: Q16836-1: 34294 Da (predicted); Q16836-2: 42140 Da (predicted); Q16836-3: 36051 Da (predicted).
    Succinylation at Lys-81, adjacent to a coenzyme A binding site

  • Subunit

    Homodimer (PubMed:10231530, PubMed:10840044, PubMed:16725361)

  • SwissProt ID

    Q16836

  • Gene ID
    3033 [NCBI]
  • Synonyms

    3 hydroxyacyl Coenzyme A dehydrogenase antibody; HAD antibody; HADH antibody; HADH1 antibody; HADHSC antibody; HADHSC, formerly antibody; HADSC, formerly antibody; HCDH antibody; HCDH_HUMAN antibody; HHF4 antibody; 3 hydroxyacyl Coenzyme A dehydrogenase antibody; HAD antibody; HADH antibody; HADH1 antibody; HADHSC antibody; HADHSC, formerly antibody; HADSC, formerly antibody; HCDH antibody; HCDH_HUMAN antibody; HHF4 antibody; Hydroxyacyl CoA dehydrogenase antibody; Hydroxyacyl-coenzyme A dehydrogenase antibody; hydroxyacyl-coenzyme A dehydrogenase, mitochondrial antibody; L 3 hydroxyacyl Coenzyme A dehydrogenase short chain antibody; M SCHAD antibody; Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase antibody; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase antibody; MGC8392 antibody; mitochondrial antibody; MSCHAD antibody; OTTHUMP00000162626 antibody; OTTHUMP00000219688 antibody; SCHAD antibody; SCHAD, formerly antibody; Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial antibody; short chain 3-hydroxyacyl-coa dehydrogenase antibody; Short-chain 3-hydroxyacyl-CoA dehydrogenase antibody;

HADH Antibody (YA6588) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using HADH Antibody (HY-P86895) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • HADH Antibody (YA6588) Product Image
    1/16
  • Experimental Validation Results for HADH Antibody (YA6588)
  • HADH Antibody (YA6588) Product Image
  • Experimental Validation Results for HADH Antibody (YA6588)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using HADH Antibody (HY-P86895) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HADH Antibody (YA6588)
  • HADH Antibody (YA6588) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using HADH Antibody (HY-P86895) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • HADH Antibody (YA6588) Product Image WB