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CD147 Antibody

(Synonyms: HT-7; CD147; EMMPRIN; AI115436; AI325119)
Customer Review

Based on 1 Customer Validation

CD147 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD147.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, IP

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • CD147 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20 1:50-1:100

Product Details

Description

CD147 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD147.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 38-58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 12215 Mouse

SwissProt: P18572 Mouse

Immunogen

Synthetic peptide corresponding to Mouse CD147.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102621

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD147 Antibody
    Western blot analysis of extracts from Hela(lane 2(20ug) , C2C12(lane 3(20ug) using CD147 Antibody (HY-P80596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD147 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD147 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD147 Antibody
    Flow cytometric analysis of 1X106 NIH-3T3 cells labeling CD147 Antibody(HY-P80596, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Essential for normal retinal maturation and development (PubMed:10967074, PubMed:11273674, PubMed:11853760). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687); Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (By similarity). Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane (PubMed:12601063). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (By similarity). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells (By similarity). Plays an important role in spermatogenesis; mediates interactions between germ cells and Sertoli cell and is essential for the development/differentiation of germ cells to round spermatids (PubMed:11882021, PubMed:23727514)

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Photoreceptor inner segment; Cell projection, cilium, photoreceptor outer segment; Cell membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Basolateral cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Retina-specific (PubMed:11273674, PubMed:11853760, PubMed:12939332, PubMed:25957687) . Expressed in both rods and cones (at protein level) (PubMed:25957687) ; Testis and caput, corpus and cauda epididymides (at protein level) (PubMed:11882021, PubMed:21792931, PubMed:23727514) . Expressed in the brain, lung, liver, kidney, heart, spleen, uterus, retina and skeletal muscle (PubMed:12601063, PubMed:12939332, PubMed:2361961)

    Induction:By estrogen in the uterine epithelium of ovariectomized animals. By eCG in ovary

  • Isoforms & Post-Translational Modification

    P18572 has 2 isomers: P18572-1: 42445 Da (predicted); P18572-2: 29674 Da (predicted).
    N-glycosylated;N-glycosylated (PubMed:12558975, PubMed:23727514). During spermatogenesis, probably deglycosylated during epididymal transit (PubMed:11882021)

  • Subunit

    Interacts with NXNL1 (PubMed:25957687). Interacts with SLC2A1 and SLC16A1/GLUT1 (By similarity). Interacts with XKR8; promoting its localization at the cell membrane (PubMed:27503893)

  • SwissProt ID

    P18572

  • Gene ID
    12215 [NCBI]
  • Synonyms

    HT-7; CD147; EMMPRIN; AI115436; AI325119

  • Research Field

    Immunology

CD147 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , C2C12(lane 3(20ug) using CD147 Antibody (HY-P80596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH-3T3 cells labeling CD147 Antibody(HY-P80596, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD147 Antibody Product Image
    1/16
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • CD147 Antibody Product Image
  • Experimental Validation Results for CD147 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , C2C12(lane 3(20ug) using CD147 Antibody (HY-P80596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD147 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD147 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD147 Antibody
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH-3T3 cells labeling CD147 Antibody(HY-P80596, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • Experimental Validation Results for CD147 Antibody
  • CD147 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , C2C12(lane 3(20ug) using CD147 Antibody (HY-P80596) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD147 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD147 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using CD147 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD147 Antibody Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 NIH-3T3 cells labeling CD147 Antibody(HY-P80596, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD147 Antibody Product Image WB
  • CD147 Antibody Product Image IHC-P
  • CD147 Antibody Product Image IHC-P
  • CD147 Antibody Product Image FC