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beta III Tubulin Antibody (YA4243)

(Synonyms: tubulin, beta 3; MC1R; TUBB4)
Customer Review

Based on 1 Customer Validation

beta III Tubulin Antibody (YA4243) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TUBB3. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • beta III Tubulin Antibody (YA4243) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

beta III Tubulin Antibody (YA4243) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TUBB3. It can be used as a loading control antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Purified recombinant fragment of human TUBB3 full(aa 1-450).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718933

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    Western blot analysis of extracts from RAW264.7 (lane 2(20μg), SH-SY5Y (lane 3(20μg), Neuro-2a (lane 4(20μg), Jurkat (lane 5(20μg) using beta III Tubulin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    Flow Cytometry analysis of MCF-7 cells labelling beta III Tubulin (red) with beta III Tubulin Antibody (anti-beta III Tubulin) (HY-P84546). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    Immunocytochemistry analysis of HEK293 cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    Immunocytochemistry analysis of Neuro-2a cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    beta III Tubulin is a Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Plays a role in dorsal root ganglion axon projection towards the spinal cord[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell projection, growth cone; Cell projection, lamellipodium; Cell projection, filopodium

  • Expression


    Tissue_specificity:This gene is primarily expressed in the central and peripheral nervous systems, and its expression is significantly enhanced in most cancerous tissues.

  • Isoforms & Post-Translational Modification

    Q13509 has 2 isomers: Q13509-1: 50433 Da (predicted); Q13509-2: 42433 Da (predicted).
    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity);Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility. Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally;Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules

  • Subunit

    Heterodimer of alpha- and beta-tubulin (PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661).

  • SwissProt ID

    Q13509

  • Gene ID
    10381 [NCBI]
  • Synonyms

    tubulin, beta 3; MC1R; TUBB4

beta III Tubulin Antibody (YA4243) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane 2(20μg), SH-SY5Y (lane 3(20μg), Neuro-2a (lane 4(20μg), Jurkat (lane 5(20μg) using beta III Tubulin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow Cytometry analysis of MCF-7 cells labelling beta III Tubulin (red) with beta III Tubulin Antibody (anti-beta III Tubulin) (HY-P84546). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HEK293 cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • beta III Tubulin Antibody (YA4243) Product Image
    1/16
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • beta III Tubulin Antibody (YA4243) Product Image
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane 2(20μg), SH-SY5Y (lane 3(20μg), Neuro-2a (lane 4(20μg), Jurkat (lane 5(20μg) using beta III Tubulin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    1/1
    MedChemExpress Validation
     Flow Cytometry analysis of MCF-7 cells labelling beta III Tubulin (red) with beta III Tubulin Antibody (anti-beta III Tubulin) (HY-P84546). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HEK293 cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Neuro-2a cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • Experimental Validation Results for beta III Tubulin Antibody (YA4243)
  • beta III Tubulin Antibody (YA4243) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7 (lane 2(20μg), SH-SY5Y (lane 3(20μg), Neuro-2a (lane 4(20μg), Jurkat (lane 5(20μg) using beta III Tubulin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • beta III Tubulin Antibody (YA4243) Product Image
    MedChemExpress Validation

    Flow Cytometry analysis of MCF-7 cells labelling beta III Tubulin (red) with beta III Tubulin Antibody (anti-beta III Tubulin) (HY-P84546). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P807578, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • beta III Tubulin Antibody (YA4243) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HEK293 cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • beta III Tubulin Antibody (YA4243) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Neuro-2a cells labeling beta III Tubulin Antibody (HY-P84546) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with beta III Tubulin Antibody (HY-P84546) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • beta III Tubulin Antibody (YA4243) Product Image WB
  • beta III Tubulin Antibody (YA4243) Product Image FC
  • beta III Tubulin Antibody (YA4243) Product Image ICC
  • beta III Tubulin Antibody (YA4243) Product Image ICC