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GATA2+GATA3 Antibody (YA6581)

(Synonyms: DCML antibody; Endothelial transcription factor GATA 2 antibody; Endothelial transcription factor GATA-2 antibody; GATA binding factor 3 antibody; GATA binding protein 2 antibody; GATA binding protein 3 antibody; GATA-binding protein 2 antibody; GATA-binding protein 3 antibody; HDR antibody; HDRS antibody; DCML antibody; Endothelial transcription factor GATA 2 antibody; Endothelial transcription factor GATA-2 antibody; GATA binding factor 3 antibody; GATA binding protein 2 antibody; GATA binding protein 3 antibody; GATA-binding protein 2 antibody; GATA-binding protein 3 antibody; HDR antibody; HDRS antibody; IMD21 antibody; MONOMAC antibody; NFE1B antibody; Trans acting T cell specific transcription factor GATA 3 antibody; Trans acting T cell specific transcription factor GATA-3 antibody; )
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Based on 1 Customer Validation

GATA2+GATA3 Antibody (YA6581) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GATA2+GATA3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GATA2+GATA3 Antibody (YA6581)
  • GATA2+GATA3 Antibody (YA6581) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:200-1:1000

Product Details

Description

GATA2+GATA3 Antibody (YA6581) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GATA2+GATA3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Immunogen

Recombinant protein within

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GATA2+GATA3 Antibody (YA6581)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using GATA2+GATA3 Antibody (HY-P86888) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    GATA2+GATA3 transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_Specificity: Endothelial cells

  • Isoforms & Post-Translational Modification

    P23769 has two isomers: P23769-1: 50500 Da (predicted); P23769-2: 49106 Da (predicted).

  • Subunit

    Interacts with BRD3 (By similarity)

  • SwissProt ID

    P23769

  • Gene ID
    2624 [NCBI]
  • Synonyms

    DCML antibody; Endothelial transcription factor GATA 2 antibody; Endothelial transcription factor GATA-2 antibody; GATA binding factor 3 antibody; GATA binding protein 2 antibody; GATA binding protein 3 antibody; GATA-binding protein 2 antibody; GATA-binding protein 3 antibody; HDR antibody; HDRS antibody; DCML antibody; Endothelial transcription factor GATA 2 antibody; Endothelial transcription factor GATA-2 antibody; GATA binding factor 3 antibody; GATA binding protein 2 antibody; GATA binding protein 3 antibody; GATA-binding protein 2 antibody; GATA-binding protein 3 antibody; HDR antibody; HDRS antibody; IMD21 antibody; MONOMAC antibody; NFE1B antibody; Trans acting T cell specific transcription factor GATA 3 antibody; Trans acting T cell specific transcription factor GATA-3 antibody;

GATA2+GATA3 Antibody (YA6581) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using GATA2+GATA3 Antibody (HY-P86888) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • GATA2+GATA3 Antibody (YA6581) Product Image
    1/16
  • Experimental Validation Results for GATA2+GATA3 Antibody (YA6581)
  • GATA2+GATA3 Antibody (YA6581) Product Image
  • Experimental Validation Results for GATA2+GATA3 Antibody (YA6581)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using GATA2+GATA3 Antibody (HY-P86888) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GATA2+GATA3 Antibody (YA6581)
  • GATA2+GATA3 Antibody (YA6581) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using GATA2+GATA3 Antibody (HY-P86888) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • GATA2+GATA3 Antibody (YA6581) Product Image WB