Niemann Pick C2 Antibody (YA1399)(PBS only)

Cat. No.: HY-P81654A: Data Sheet User Guide for Antibodies
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Niemann Pick C2 Antibody (YA1399) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Niemann Pick C2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Niemann Pick C2 Antibody (YA1399) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Niemann Pick C2.

Host

Rabbit

Molecular Weight

Predicted band size: 17 kDa;

Observed band size: 17 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P61916

Gene ID

10577 [NCBI]

Immunogen

Recombinant protein of human Niemann Pick C2 aa20-151.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	500-1:2000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:11125141, PubMed:15937921, PubMed:17018531, PubMed:18772377, PubMed:29580834). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed:17018531, PubMed:18772377, PubMed:27238017). May bind and mobilize cholesterol that is associated with membranes (PubMed:18823126). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:17018531). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed:17018531). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity)

Subcellular Localization：Secreted; Endoplasmic reticulum; Lysosome

Expression：
Tissue_specificity:Detected in gallbladder bile (PubMed:21315718) . Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level) (PubMed:11125141) . Epididymis

Induction:Down-regulated in response to enterovirus 71 (EV71) infection

Isoforms & Post-Translational Modification：P61916 has 2 isomers: P61916-1: 16570 Da (predicted); P61916-2: 13416 Da (predicted).

Subunit：Interacts with NPC1 (via the second lumenal domain) in a cholestrol-dependent manner (PubMed:18772377, PubMed:27238017, PubMed:27551080).

Research Field

Cardiovascular

Synonyms

HE1; EDDM1

Documentation

User Guide for Antibodies (1077 KB)