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CHD3 Antibody (YA945)

(Synonyms: CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH)
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Based on 1 Customer Validation

CHD3 Antibody (YA945) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • CHD3 Antibody (YA945) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

CHD3 Antibody (YA945) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHD3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 260 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 227 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CHD3 aa220-300.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CHD3 Antibody (YA945)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), and Jurkat (lane 3, 15 μg) using CHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CHD3 Antibody (YA945)
    Western blot analysis of extracts from K562 (lane 2(20μg), SiHa (lane 3(20μg), Jurkat (lane 4(20μg), NIH/3T3 (lane 5(20μg) using CHD3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CHD3 Antibody (YA945)
    Flow Cytometry analysis of Hela cells labelling CHD3 (red) with CHD3 Antibody (anti-CHD3) (HY-P81264A). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CHD3 Antibody (YA945)
    Immunocytochemistry analysis of HeLa cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CHD3 Antibody (YA945)
    Immunocytochemistry analysis of C6 cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CHD3 is an ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity[1][2][3][4][5][6].

  • Subcellular Localization

    Nucleus, PML body; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Expression


    Tissue_specificity:Broad expression

  • Isoforms & Post-Translational Modification

    Q12873 has 3 isomers: Q12873-1: 226592 Da (predicted); Q12873-2: 222861 Da (predicted); Q12873-3: 233037 Da (predicted).
    Sumoylation at Lys-1971 results in dissociation from chromatin and suppression of transcriptional repression

  • Subunit

    Component of the nucleosome remodeling and deacetylase (NuRD) repressor complex, composed of core proteins MTA1, MTA2, MTA3, RBBP4, RBBP7, HDAC1, HDAC2, MBD2, MBD3, and peripherally associated proteins CDK2AP1, CDK2AP2, GATAD2A, GATAD2B, CHD3, CHD4 and CHD5 (PubMed:16428440, PubMed:27068747, PubMed:28977666, PubMed:33283408, PubMed:9804427). The exact stoichiometry of the NuRD complex is unknown, and some subunits such as MBD2 and MBD3, GATAD2A and GATAD2B, and CHD3, CHD4 and CHD5 define mutually exclusive NuRD complexes (PubMed:16428440, PubMed:28977666, PubMed:33283408). Interacts with CBX1 (PubMed:28977666). Interacts with CBX3 (PubMed:28977666). Interacts with CBX5 (PubMed:28977666). Interacts (via its C-terminal) with HABP4 (PubMed:12505151). Interacts with HDAC1 (PubMed:28977666). Interacts with MTA1 (PubMed:28977666). Interacts with PCNT; the interaction regulates centrosome integrity (PubMed:17626165). Interacts with RBBP7 (PubMed:28977666). Interacts with SERBP1 (PubMed:12505151). Interacts with TRIM28 (PubMed:11230151). Interacts with ZBED1/hDREF (PubMed:27068747)

  • SwissProt ID

    Q12873

  • Gene ID
    1107 [NCBI]
  • Synonyms

    CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH

  • Research Field

    Immunology

CHD3 Antibody (YA945) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), and Jurkat (lane 3, 15 μg) using CHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), SiHa (lane 3(20μg), Jurkat (lane 4(20μg), NIH/3T3 (lane 5(20μg) using CHD3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow Cytometry analysis of Hela cells labelling CHD3 (red) with CHD3 Antibody (anti-CHD3) (HY-P81264A). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CHD3 Antibody (YA945) Product Image
    1/16
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • CHD3 Antibody (YA945) Product Image
  • Experimental Validation Results for CHD3 Antibody (YA945)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), and Jurkat (lane 3, 15 μg) using CHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CHD3 Antibody (YA945)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), SiHa (lane 3(20μg), Jurkat (lane 4(20μg), NIH/3T3 (lane 5(20μg) using CHD3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CHD3 Antibody (YA945)
    1/1
    MedChemExpress Validation
     Flow Cytometry analysis of Hela cells labelling CHD3 (red) with CHD3 Antibody (anti-CHD3) (HY-P81264A). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CHD3 Antibody (YA945)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CHD3 Antibody (YA945)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • Experimental Validation Results for CHD3 Antibody (YA945)
  • CHD3 Antibody (YA945) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), HepG2 (lane 2, 15 μg), and Jurkat (lane 3, 15 μg) using CHD3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CHD3 Antibody (YA945) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), SiHa (lane 3(20μg), Jurkat (lane 4(20μg), NIH/3T3 (lane 5(20μg) using CHD3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CHD3 Antibody (YA945) Product Image
    MedChemExpress Validation

    Flow Cytometry analysis of Hela cells labelling CHD3 (red) with CHD3 Antibody (anti-CHD3) (HY-P81264A). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CHD3 Antibody (YA945) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CHD3 Antibody (YA945) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling CHD3 with CHD3 Antibody (HY-P81264A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHD3 Antibody (HY-P81264A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CHD3 Antibody (YA945) Product Image WB
  • CHD3 Antibody (YA945) Product Image WB
  • CHD3 Antibody (YA945) Product Image FC
  • CHD3 Antibody (YA945) Product Image ICC
  • CHD3 Antibody (YA945) Product Image ICC