SPR Antibody (YA6777)

Cat. No.: HY-P87084: Fiche technique COA
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SPR Antibody (YA6777) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPR.

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Size	Stock	Quantité
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

SPR Antibody (YA6777) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPR.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Masse moléculaire

Predicted band size: 28 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P35270

Gene ID

6697 [NCBI]

Immunogen

Recombinant protein within Human SPR aa 1-261.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50
FC FC: Flow Cytometry	1:1000

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HepG2 (lane 4), HeLa (lane 5), and A549 (lane 6) using SPR antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin

Subcellular Localization：Cytoplasm

Isoforms & Post-Translational Modification：P35270: 261 amino acids, molecular weight 28048 Da.

Subunit：Homodimer

Synonyms

OTTHUMP00000160199 antibody; SDR38C1 antibody; Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody; Sepiapterin reductase antibody; Short chain dehydrogenase/reductase family 38C, member 1 antibody; SPR antibody; SPRE_HUMAN antibody;

Documentation

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Fiche technique (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)