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SPR Antibody (YA6777)

(Synonyms: OTTHUMP00000160199 antibody; SDR38C1 antibody; Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody; Sepiapterin reductase antibody; Short chain dehydrogenase/reductase family 38C, member 1 antibody; SPR antibody; SPRE_HUMAN antibody; )
Customer Review

Based on 1 Customer Validation

SPR Antibody (YA6777) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SPR Antibody (YA6777)
  • SPR Antibody (YA6777) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:2000 1:50 1:1000

Product Details

Description

SPR Antibody (YA6777) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SPR.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Immunogen

Recombinant protein within Human SPR aa 1-261.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SPR Antibody (YA6777)
    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HepG2 (lane 4), HeLa (lane 5), and A549 (lane 6) using SPR antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    SPR catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    P35270: 261 amino acids, molecular weight 28048 Da.

  • Subunit

    Homodimer

  • SwissProt ID

    P35270

  • Gene ID
    6697 [NCBI]
  • Synonyms

    OTTHUMP00000160199 antibody; SDR38C1 antibody; Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody; Sepiapterin reductase antibody; Short chain dehydrogenase/reductase family 38C, member 1 antibody; SPR antibody; SPRE_HUMAN antibody;

SPR Antibody (YA6777) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HepG2 (lane 4), HeLa (lane 5), and A549 (lane 6) using SPR antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • SPR Antibody (YA6777) Product Image
    1/16
  • Experimental Validation Results for SPR Antibody (YA6777)
  • SPR Antibody (YA6777) Product Image
  • Experimental Validation Results for SPR Antibody (YA6777)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HepG2 (lane 4), HeLa (lane 5), and A549 (lane 6) using SPR antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for SPR Antibody (YA6777)
  • SPR Antibody (YA6777) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HepG2 (lane 4), HeLa (lane 5), and A549 (lane 6) using SPR antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • SPR Antibody (YA6777) Product Image WB