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ATP6V1A Antibody (YA2211)

(Synonyms: HO68; VA68; VPP2; Vma1; ARCL2D; ATP6A1; IECEE3; ATP6V1A1)
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Based on 1 Customer Validation

ATP6V1A Antibody (YA2211) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V1A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATP6V1A Antibody (YA2211)
  • ATP6V1A Antibody (YA2211) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

ATP6V1A Antibody (YA2211) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP6V1A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human ATP6V1A aa62-78.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104574

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP6V1A Antibody (YA2211)
    Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg),3T3 (lane 4(20μg), using ATP6V1A Antibody (YA2211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ATP6V1A is a Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity; (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytosol; Cytoplasmic vesicle, secretory vesicle; Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Lysosome

  • Expression


    Tissue_specificity:High expression in the skin

  • Isoforms & Post-Translational Modification

    P38606 has 2 isomers: P38606-1: 68304 Da (predicted); P38606-2: 64736 Da (predicted).
    Phosphorylation at Ser-384 by AMPK down-regulates its enzyme activity

  • Subunit

    V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex (PubMed:33065002).

  • SwissProt ID

    P38606

  • Gene ID
    523 [NCBI]
  • Synonyms

    HO68; VA68; VPP2; Vma1; ARCL2D; ATP6A1; IECEE3; ATP6V1A1

  • Research Field

    Tags & Cell Markers

ATP6V1A Antibody (YA2211) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg),3T3 (lane 4(20μg), using ATP6V1A Antibody (YA2211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ATP6V1A Antibody (YA2211) Product Image
    1/16
  • Experimental Validation Results for ATP6V1A Antibody (YA2211)
  • ATP6V1A Antibody (YA2211) Product Image
  • Experimental Validation Results for ATP6V1A Antibody (YA2211)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg),3T3 (lane 4(20μg), using ATP6V1A Antibody (YA2211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP6V1A Antibody (YA2211)
  • ATP6V1A Antibody (YA2211) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), K562 (lane 3(20μg),3T3 (lane 4(20μg), using ATP6V1A Antibody (YA2211). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ATP6V1A Antibody (YA2211) Product Image WB