PAK3 Antibody (YA3567)(PBS only)

Cat. No.: HY-P83870A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PAK3 Antibody (YA3567) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PAK3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PAK3 Antibody (YA3567) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PAK3.

Host

Mouse

Molecular Weight

Predicted band size: 62 kDa;

Observed band size: 62 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

O75914

Gene ID

5063 [NCBI]

Immunogen

Purified recombinant fragment of human PAK3 (AA: 1-100) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Limited to the nervous system. Highly expressed in postmitotic neurons of the cerebral cortex and hippocampus during development and after birth.

Isoforms & Post-Translational Modification：O75914 has 4 isomers: O75914-1: 62310 Da (predicted); O75914-2: 60693 Da (predicted); O75914-3: 64530 Da (predicted); O75914-4: 62913 Da (predicted).
Autophosphorylated when activated by CDC42/p21;Neddylated

Subunit：Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK.

Synonyms

ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK

Documentation

User Guide for Antibodies (1077 KB)