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  5. Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333)

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333)

Art. -Nr.: HY-P83588
COA
SDS
User Guide for Antibodies Technical Support

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4/5/6 (Ser474/Ser560/Ser602).

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

Beschreibung

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4/5/6 (Ser474/Ser560/Ser602).
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molekulargewicht
Predicted band size: 64,80,75 kDa;
Observed band size: 64,80,75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602)
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Reinheit Affinity Purified
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela(lane 2(20ug) , A549(lane 3(20ug) and HL-60(lane 4(20ug) using Phospho-PAK4/5/6 Antibody (HY-P83588) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：O96013: Serine/threonine-protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell adhesion turnover, cell migration, growth, proliferation or cell survival (PubMed:26598620). Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin.
Q9P286: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions
Q9NQU5: Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Also inhibits ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD
Subcellular Localization：O96013: Cytoplasm
Q9P286: Mitochondrion; Cytoplasm; Nucleus
Q9NQU5: Cytoplasm; Nucleus
Expression：
Tissue_specificity: O96013: Highest expression in prostate, testis and colon
Q9P286: Predominantly expressed in brain
Q9NQU5: Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast
Subunit：O96013: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1 (PubMed:15827085, PubMed:26598620). Interacts with INKA1 (PubMed:26607847)
Q9P286: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH
Q9NQU5: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B
RRID
Database

Entrez Gene: 10298 Human ; 57144 Human ; 56924 Human

SwissProt: O96013 Human ; Q9P286 Human ; Q9NQU5 Human

Research Field

Signal Transduction
Synonyms
PAK4; PAK5; PAK6
Dokumentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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