Toll-like Receptor 7 Antibody (YA5330)

Cat. No.: HY-P85638: Data Sheet User Guide for Antibodies
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Toll-like Receptor 7 Antibody (YA5330) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Toll-like Receptor 7.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Toll-like Receptor 7 Antibody (YA5330) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Toll-like Receptor 7.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 121 kDa;

Species Reactivity

Transfected

SwissProt ID

Q9NYK1

Gene ID

51284 [NCBI]

Immunogen

Purified recombinant human Toll-like Receptor protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Sensitivity	Transfected	Purity	affinity chromatography.
Conjugation	Non-conjugated	Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed:14976261, PubMed:32433612). Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs (PubMed:12738885, PubMed:27742543, PubMed:31608988, PubMed:32706371, PubMed:35477763). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed:27742543). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed:27742543, PubMed:32706371). In plasmacytoid dendritic cells, RNASET2 endonuclease cooperates with PLD3 or PLD4 5'->3' exonucleases to process RNA and release 2',3'-cyclic guanosine monophosphate (2',3'-cGMP) and cytidine-rich RNA fragments that occupy TLR7 ligand-binding pockets and trigger a signaling-competent state

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type I membrane protein; Endosome; Lysosome; Cytoplasmic vesicle, phagosome

Expression：
Tissue_specificity:It was detected in the brain, placenta, spleen, stomach, small intestine, lungs, and plasmacytoid anterior dendritic cells. It is also expressed in peripheral blood mononuclear cells (PubMed: 32706371) .

Subunit：Homodimer (PubMed:27742543). Interacts with MYD88 via their respective TIR domains (Probable). Interacts with UNC93B1 (PubMed:33432245). Interacts with SMPDL3B (By similarity)

Synonyms

PRO285; TLR 7; Tlr7; TLR7_HUMAN; Toll like receptor 7; Toll-like receptor 7; UNQ248.

Documentation

User Guide for Antibodies (1077 KB)