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CSK Antibody (YA2742)

(Synonyms: CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL)
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Based on 1 Customer Validation

CSK Antibody (YA2742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CSK.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CSK Antibody (YA2742)
  • Experimental Validation Results for CSK Antibody (YA2742)
  • CSK Antibody (YA2742) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

CSK Antibody (YA2742) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CSK.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CSK aa50-100/450.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105115

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CSK Antibody (YA2742)
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CSK Antibody (YA2742)
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CSK (C-terminal Src kinase) is a non-receptor tyrosine kinase that functions as a central negative regulator of Src family kinases (SFKs) through phosphorylation of their conserved C-terminal inhibitory tyrosine residue, thereby maintaining controlled intracellular tyrosine kinase signaling and suppressing excessive SFK activity[1]. Mechanistically, CSK is recruited to membrane-associated signaling complexes through interactions mediated by its SH2 domain, and this localization enables efficient regulation of SFK-dependent pathways involved in cell proliferation, differentiation, migration, immune signaling, and survival[1][2]. Disruption of the CSK-centered regulatory network, including alterations in scaffold proteins such as Cbp/PAG1 and associated phosphatase systems, can result in sustained SFK activation, a molecular feature frequently observed in cancer and other pathological conditions[1]. Experimental studies further demonstrate that loss of CSK function causes constitutive activation of SFKs in vivo, highlighting its essential role in maintaining signaling homeostasis during development and tissue regulation[1]. Compared with the closely related isoform CHK (CSK-homologous kinase), CSK functions as a highly specific kinase for the inhibitory C-terminal regulatory site of SFKs, whereas CHK exhibits distinct inhibitory properties and mechanistic characteristics despite sharing the common role of SFK suppression[3]. For experimental applications, CSK is widely used as a molecular tool for investigating SFK signaling, kinase regulation, immune receptor pathways, and oncogenic tyrosine kinase networks, while small-molecule CSK inhibitors remain relatively limited compared with the extensive pharmacological targeting of SFKs themselves[2][4].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:Expressed in lung and macrophage cells

  • Subunit

    Homodimer (via SH3-domain) (PubMed:19888460). Interacts with PTPN22 (PubMed:15208781). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases (PubMed:10790433, PubMed:10838081, PubMed:11433379, PubMed:14610046). Interacts with SRCIN1 (PubMed:17525734). Interacts with RHOH (PubMed:20851766). Interacts (via SH2 domain) with SCIMP; this interaction is dependent on phosphorylation of SCIMP 'Tyr-107' (PubMed:21930792). Interacts (via SH2 domain) with PRAG1 (when phosphorylated at 'Tyr-391'); this interaction prevents translocation of CSK from the cytoplasm to the membrane leading to increased activity of CSK (By similarity). Interacts with LRRK1 (PubMed:23526378)

  • SwissProt ID

    P41240

  • Gene ID
    1445 [NCBI]
  • Synonyms

    CSK; Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL

  • Research Field

    Signal Transduction

[1]. Okada M. Regulation of the SRC family kinases by Csk. Int J Biol Sci. 2012;8(10):1385-97. doi: 10.7150/ijbs.5141. Epub 2012 Nov 1. PMID: 23139636; PMCID: PMC3492796. et al. Regulation of the SRC family kinases by Csk. Int J Biol Sci. 2012;8(10):1385-97. [Content Brief]

[2]. Zhu S, et al. Regulation, targets and functions of CSK. Front Cell Dev Biol. 2023 Jun 16;11:1206539. [Content Brief]

[3]. Advani G, et al. Csk-homologous kinase (Chk) is an efficient inhibitor of Src-family kinases but a poor catalyst of phosphorylation of their C-terminal regulatory tyrosine. Cell Commun Signal. 2017 Aug 7;15(1):29. [Content Brief]

[4]. Database: Guide to pharmacology.

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CSK Antibody (YA2742) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CSK Antibody (YA2742) Product Image
    1/16
  • Experimental Validation Results for CSK Antibody (YA2742)
  • Experimental Validation Results for CSK Antibody (YA2742)
  • CSK Antibody (YA2742) Product Image
  • Experimental Validation Results for CSK Antibody (YA2742)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CSK Antibody (YA2742)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CSK Antibody (YA2742)
  • Experimental Validation Results for CSK Antibody (YA2742)
  • CSK Antibody (YA2742) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CSK Antibody (YA2742) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using CSK Antibody (YA2742). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82997, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CSK Antibody (YA2742) Product Image IHC-P
  • CSK Antibody (YA2742) Product Image IHC-P