GlyT2 Antibody (YA2936)
(Synonyms: NET1; GLYT2; HKPX3; GLYT-2)
Based on 1 Customer Validation
GlyT2 Antibody (YA2936) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GlyT2.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB
-
Reactivity :
Human, Rat
-
Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
GlyT2 Antibody (YA2936) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GlyT2.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman, Rat
-
Observed Molecular WeightObserved band size: 87 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 87 kDa
Entrez Gene: 9152 Human ; 171148 Rat
SwissProt: Q9Y345 Human ; Q761V0 Mouse ; P58295 Rat
OMIM: 614618 Human
A synthetic peptide of human Glyt2
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Verification Images
-
Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), U937 (lane 2, 15 μg), U-87MG (lane 3, 15 μg), U251MG (lane 4, 15 μg), C6 (lane 5, 15 μg), and Mouse brain (lane 6, 15 μg) using Glyt2 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C. -
Western blot analysis of extracts from Jurkat (lane 2(20μg), C6(lane 3(20μg), SH-SY5Y(lane 4(20μg) and U937(lane 5(20μg) using GlyT2 Antibody (HY-P83191). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
-
Function
Glycine transporter 2 (GlyT2), encoded by SLC6A5, is a presynaptic sodium- and chloride-dependent transporter that recaptures glycine into glycinergic nerve terminals and maintains the intracellular glycine pool required for efficient inhibitory neurotransmission[1][2]. Mechanistically, GlyT2 preserves vesicular glycine content by supplying substrate for vesicle refilling, thereby sustaining quantal glycinergic signaling in the spinal cord, brainstem, retina, and other glycinergic circuits[1][3]. GlyT2-mediated transport is characterized by high-affinity glycine uptake coupled to Na+ and Cl− gradients, a process that is essential for maintaining presynaptic glycine availability and synaptic inhibitory strength[2]. Regulation of GlyT2 expression also links glycinergic transmission to broader signaling networks, including Hedgehog pathway activity, which can modulate GlyT2 abundance, transport activity, and glycine homeostasis through ubiquitination-dependent mechanisms[3]. In disease contexts, loss-of-function mutations in SLC6A5 cause hyperekplexia, and GlyT2-deficient animal models develop severe motor abnormalities, impaired inhibitory transmission, and early postnatal lethality, highlighting the transporter’s critical physiological role[1][2][3]. Compared with the related isoform GlyT1, which is predominantly associated with glial glycine clearance around synapses, GlyT2 is localized to glycinergic presynaptic terminals and is required for maintaining transmitter stores rather than primarily shaping extracellular glycine levels[2][4]. For experimental applications, selective GlyT2 inhibitors such as ALX-1393 and ORG25543 are widely used to investigate glycinergic signaling, transporter selectivity, and glycine-dependent modulation of neuronal circuits[4].
-
Subcellular Localization
Cell membrane; Multi-pass membrane protein
-
Expression
Tissue_specificity:Expressed in medulla, and to a lesser extent in spinal cord and cerebellum -
Isoforms & Post-Translational Modification
Q9Y345 has 3 isomers: Q9Y345-1: 87434 Da (predicted); Q9Y345-2: 63363 Da (predicted); Q9Y345-3: 85860 Da (predicted).
N-glycosylated -
SwissProt ID
-
Synonyms
NET1; GLYT2; HKPX3; GLYT-2
-
Research Field
Neuroscience
Documentation
-
Data Sheet (261 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
User Guide for Antibodies (1077 KB)
References
[1]. Zafra F, et al. Glycinergic transmission: glycine transporter GlyT2 in neuronal pathologies. Neuronal Signal. 2016 Dec 22;1(1):NS20160009. [Content Brief]
[3]. de la Rocha-Muñoz A, et al. The presynaptic glycine transporter GlyT2 is regulated by the Hedgehog pathway in vitro and in vivo. Commun Biol. 2021 Oct 18;4(1):1197. [Content Brief]
[4]. Łątka K, et al. Analysis of Binding Determinants for Different Classes of Competitive and Noncompetitive Inhibitors of Glycine Transporters. Int J Mol Sci. 2022 Jul 21;23(14):8050. [Content Brief]
Powered by Bioz
