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PPP2R5E Antibody (YA1708)

(Synonyms: B56E; B56epsilon)
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Based on 1 Customer Validation

PPP2R5E Antibody (YA1708) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPP2R5E.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PPP2R5E Antibody (YA1708)
  • PPP2R5E Antibody (YA1708) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:200-1:500 1:200-1:500 1:200-1:1000 1:50-1:100

Product Details

Description

PPP2R5E Antibody (YA1708) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PPP2R5E.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PPP2R5E

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PPP2R5E Antibody (YA1708)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Mouse spleen (lane 3), Rat brain (lane 4), HeLa (lane 5), 293T (lane 6), and NIH/3T3 (lane 7) using PPP2R5E antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    PPP2R5E is a The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Q16537 has 3 isomers: Q16537-1: 54699 Da (predicted); Q16537-2: 54043 Da (predicted); Q16537-3: 46180 Da (predicted).
    Phosphorylated on serine residues

  • Subunit

    Found in a complex with at least ARL2, PPP2CB; PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity).

  • SwissProt ID

    Q16537

  • Gene ID
    5529 [NCBI]
  • Synonyms

    B56E; B56epsilon

  • Research Field

    Neuroscience

PPP2R5E Antibody (YA1708) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Mouse spleen (lane 3), Rat brain (lane 4), HeLa (lane 5), 293T (lane 6), and NIH/3T3 (lane 7) using PPP2R5E antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • PPP2R5E Antibody (YA1708) Product Image
    1/16
  • Experimental Validation Results for PPP2R5E Antibody (YA1708)
  • PPP2R5E Antibody (YA1708) Product Image
  • Experimental Validation Results for PPP2R5E Antibody (YA1708)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Mouse spleen (lane 3), Rat brain (lane 4), HeLa (lane 5), 293T (lane 6), and NIH/3T3 (lane 7) using PPP2R5E antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for PPP2R5E Antibody (YA1708)
  • PPP2R5E Antibody (YA1708) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Mouse spleen (lane 3), Rat brain (lane 4), HeLa (lane 5), 293T (lane 6), and NIH/3T3 (lane 7) using PPP2R5E antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • PPP2R5E Antibody (YA1708) Product Image WB