FKBP51 Antibody (YA7225)

Cat. No.: HY-P87541: Data Sheet User Guide for Antibodies
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FKBP51 Antibody (YA7225) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FKBP51.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

FKBP51 Antibody (YA7225) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FKBP51.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 51 kDa;

Predicted Reactivity

Human,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q13451

Immunogen

KLH conjugated synthetic peptide derived from human FKBP51

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1:50-100

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Immunophilin protein with PPIase and co-chaperone activities (PubMed:11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed:12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed:28147277, PubMed:28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed:26101251, PubMed:31434731)

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Widely expressed, enriched in testis compared to other tissues
Induction: By androgen

Isoforms & Post-Translational Modification：Q13451 has two isomers: Q13451-1: 51212 Da (predicted); Q13451-2: 30032 Da (predicted).
Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1 (PubMed:28147277)丨Ubiquitinated, leading to degradation in a proteasome-dependent manner

Subunit：Part of a heteromultimeric cytoplasmic complex with HSP90AA1, HSPA1A/HSPA1B and steroid receptors

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/su-dhl-1-cell.html [Content Brief]