CTNNBL1 Antibody (YA4392)(PBS only)

Cat. No.: HY-P84695A: Data Sheet User Guide for Antibodies
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CTNNBL1 Antibody (YA4392) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CTNNBL1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CTNNBL1 Antibody (YA4392) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CTNNBL1.

Host

Mouse

Molecular Weight

Predicted band size: 65 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8WYA6

Gene ID

56259 [NCBI]

Immunogen

Purified recombinant fragment of human CTNNBL1 (AA: 390-557) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated somatic hypermutation (SHM) and class-switch recombination (CSR), 2 processes resulting in the production of high-affinity, mutated isotype-switched antibodies (PubMed:32484799)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Widely expressed with highest levels in skeletal muscle, placenta, heart, spleen, testis and thyroid

Isoforms & Post-Translational Modification：Q8WYA6 has 4 isomers: Q8WYA6-1: 65173 Da (predicted); Q8WYA6-2: 43392 Da (predicted); Q8WYA6-3: 36103 Da (predicted); Q8WYA6-4: 61971 Da (predicted).

Subunit：Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly with CWC15 and CDC5L in the complex (PubMed:32484799). Interacts with AICDA; the interaction is important for the antibody diversification activity of AICDA (PubMed:18722174, PubMed:21385873). Interacts with PRPF31 (via its NLS). Interacts (via its N-terminal NLS) with KPNA1 and KPNA2

Synonyms

NAP; P14L; PP8304; C20orf33; dJ633O20.1

Documentation

User Guide for Antibodies (1077 KB)