Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Antibody (YA6721)

Cat. No.: HY-P87028: Ficha de datos COA
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Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Antibody (YA6721) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602).

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Antibody (YA6721) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 64/81/75 kDa;

Observed band size: 64 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O96013

Gene ID

10298 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Phosphorylated	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HUVEC (lane 2(20μg)) 、MCF7 (lane 3(20μg)) and HL-60 (lane 4(20μg)) using Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Antibody (HY-P87028) . Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Serine/threonine-protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell adhesion turnover, cell migration, growth, proliferation or cell survival (PubMed:26598620). Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. Promotes kinase-independent stabilization of RHOU, thereby contributing to focal adhesion disassembly during cell migration (PubMed:26598620)

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Highest expression in prostate, testis and colon

Isoforms & Post-Translational Modification：O96013 has four isomers: O96013-1: 64072 Da (predicted); O96013-2: 47924 Da (predicted); O96013-3: 48268 Da (predicted); O96013-4: 54940 Da (predicted).
Autophosphorylated on serine residues when activated by CDC42/p21 (Ref丨Polyubiquitinated, leading to its proteasomal degradation

Subunit：Interacts with FGFR2 and GRB2 (By similarity)

Synonyms

KIAA1142 antibody; p21 activated kinase 4 antibody; p21 Cdc42/Rac1-actiated kinase 4 antibody; P21 protein (Cdc42/Rac) activated kinase 4 antibody; p21(CDKN1A) activated kinase 4 antibody; p21-activated kinase 4 antibody; PAK 4 antibody; PAK-4 antibody; Pak4 antibody; PAK4_HUMAN antibody; KIAA1142 antibody; p21 activated kinase 4 antibody; p21 Cdc42/Rac1-actiated kinase 4 antibody; P21 protein (Cdc42/Rac) activated kinase 4 antibody; p21(CDKN1A) activated kinase 4 antibody; p21-activated kinase 4 antibody; PAK 4 antibody; PAK-4 antibody; Pak4 antibody; PAK4_HUMAN antibody; Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody; Serine threonine kinase PAK 4 antibody; Serine/threonine protein kinase PAK 4 antibody; Serine/threonine protein kinase PAK4 antibody; Serine/threonine-protein kinase PAK 4 antibody; EC 2.7.11.1 antibody; KIAA1264 antibody; MGC26232 antibody; p21 activated kinase 7 antibody; p21 protein (Cdc42/Rac)-activated kinase 7 antibody; p21(CDKN1A) activated kinase 7 antibody; p21-activated kinase 5 antibody; p21-activated kinase 7 antibody; PAK 5 antibody; PAK 7 antibody; PAK-5 antibody; PAK-7 antibody; PAK5 antibody; PAK7 antibody; PAK7_HUMAN antibody; Protein kinase PAK5 antibody; Serine/threonine protein kinase PAK 7 antibody; Serine/threonine-protein kinase PAK 7 antibody; CDKN1A activated kinase 6 antibody; EC 2.7.1.37 antibody; p21 activated protein kinase 6 antibody; p21 protein (Cdc42/Rac)-activated kinase 6 antibody; p21(CDKN1A) activated kinase 6 antibody; p21-activated kinase 6 antibody; p21activated kinase 6 antibody; PAK 6 antibody; PAK-6 antibody; Pak6 antibody; PAK6_HUMAN antibody; Serine threonine protein kinase PAK 6 antibody; Serine/threonine protein kinase PAK 6 antibody; Serine/threonine protein kinase PAK6 antibody; Serine/threonine-protein kinase PAK 6 antibody;

Documentación

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COA (206 KB)

User Guide for Antibodies (1077 KB)