Calnexin Antibody (YA4764)(PBS only)

Cat. No.: HY-P85072A: Fiche technique User Guide for Antibodies
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Calnexin Antibody (YA4764) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Calnexin.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

Calnexin Antibody (YA4764) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Calnexin.

Host

Mouse

Masse moléculaire

Predicted band size: 68 kDa;

Species Reactivity

Human

SwissProt ID

P27824

Gene ID

821 [NCBI]

Immunogen

Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureté	affinity purified.	Conjugation	Non-conjugated

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type I membrane protein; Mitochondrion membrane; Single-pass type I membrane protein; Melanosome membrane; Single-pass type I membrane protein

Isoforms & Post-Translational Modification：P27824 has 3 isomers: P27824-1: 67568 Da (predicted); P27824-2: 71503 Da (predicted); P27824-3: 55598 Da (predicted).
Phosphorylated at Ser-564 by MAPK3/ERK1. Phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity);Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins;Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4

Subunit：Interacts with MAPK3/ERK1 (By similarity). Interacts with KCNH2 (PubMed:16361248). Associates with ribosomes (By similarity). Interacts with SGIP1; involved in negative regulation of endocytosis (By similarity).

Synonyms

CANX; Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90

Documentation

User Guide for Antibodies (1077 KB)