SLC40A1 Antibody

Cat. No.: HY-P81218: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

SLC40A1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLC40A1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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SLC40A1 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

SLC40A1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLC40A1.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 63 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human Predicted Reactivity: Mouse,Rat,Dog,Pig,Rabbit

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q9NP59

Gene ID

30061 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human SLC40A1/FPN1: 331-430/571

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat (lane 1) and K562 (lane 2) and HepG2 (lane 3) and HCT116 (lane 4) using SLC40A1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis (PubMed:15692071, PubMed:22178646, PubMed:22682227, PubMed:24304836, PubMed:29237594, PubMed:29599243, PubMed:30247984). Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues (By similarity). Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes (By similarity). When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma (PubMed:22682227, PubMed:29237594, PubMed:32814342)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Detected in erythrocytes (at protein level) (PubMed:23219802) . Expressed in placenta, intestine, muscle and spleen (PubMed:10747949) . Highly expressed in mature red blood (PubMed:29599243)

Subunit：Identified in a complex with STOM (PubMed:23219802). Interacts with HAMP; affinity of the peptide hormone HAMP for SLC40A1 increases by 80-fold in the presence of iron and the interaction promotes SLC40A1 ubiquitination and degradation (PubMed:22682227, PubMed:29237594, PubMed:32814342). Part of a complex composed of SLC40A1/ferroportin, TF/transferrin and HEPH/hephaestin that transfers iron from cells to transferrin (PubMed:37277838)

RRID

AB_3103280

Database

Entrez Gene: 30061 Human

SwissProt: Q9NP59 Human

OMIM: 606069 Human

Synonyms

Ferroportin 1; Ferroportin-1; FPN1; HFE4; IREG1; Iron regulated transporter 1; Iron-regulated transporter 1; MTP1; S40A1_HUMAN; SLC40A1; Solute carrier family 40 member 1; MST079; MSTP079; MTP1; SLC11A3.

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

SLC40A1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SLC40A1 Antibody

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SLC40A1 Antibody Related Classifications

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