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SLC40A1 Antibody

(Synonyms: Ferroportin 1; Ferroportin-1; FPN1; HFE4; IREG1; Iron regulated transporter 1; Iron-regulated transporter 1; MTP1; S40A1_HUMAN; SLC40A1; Solute carrier family 40 member 1; MST079; MSTP079; MTP1; SLC11A3.)
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Based on 1 Customer Validation

SLC40A1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLC40A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SLC40A1 Antibody
  • SLC40A1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-2000

Product Details

Description

SLC40A1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLC40A1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human Predicted Reactivity: Mouse,Rat,Dog,Pig,Rabbit
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Species Reactivity Database

Entrez Gene: 30061 Human

SwissProt: Q9NP59 Human

Immunogen

KLH conjugated synthetic peptide derived from human SLC40A1/FPN1: 331-430/571

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103280

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLC40A1 Antibody
    Western blot analysis of extracts from Jurkat (lane 1) and K562 (lane 2) and HepG2 (lane 3) and HCT116 (lane 4) using SLC40A1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    SLC40A1 transports Fe(2+) from the inside of a cell to the outside of the cell, playing a key role for maintaining systemic iron homeostasis. Transports iron from intestinal, splenic, hepatic cells, macrophages and erythrocytes into the blood to provide iron to other tissues. Controls therefore dietary iron uptake, iron recycling by macrophages and erythrocytes, and release of iron stores in hepatocytes. When iron is in excess in serum, circulating HAMP/hepcidin levels increase resulting in a degradation of SLC40A1, thus limiting the iron efflux to plasma[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Detected in erythrocytes (at protein level) (PubMed:23219802) . Expressed in placenta, intestine, muscle and spleen (PubMed:10747949) . Highly expressed in mature red blood (PubMed:29599243)

  • Subunit

    Identified in a complex with STOM (PubMed:23219802). Interacts with HAMP; affinity of the peptide hormone HAMP for SLC40A1 increases by 80-fold in the presence of iron and the interaction promotes SLC40A1 ubiquitination and degradation (PubMed:22682227, PubMed:29237594, PubMed:32814342). Part of a complex composed of SLC40A1/ferroportin, TF/transferrin and HEPH/hephaestin that transfers iron from cells to transferrin (PubMed:37277838)

  • SwissProt ID

    Q9NP59

  • Gene ID
    30061 [NCBI]
  • Synonyms

    Ferroportin 1; Ferroportin-1; FPN1; HFE4; IREG1; Iron regulated transporter 1; Iron-regulated transporter 1; MTP1; S40A1_HUMAN; SLC40A1; Solute carrier family 40 member 1; MST079; MSTP079; MTP1; SLC11A3.

SLC40A1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1) and K562 (lane 2) and HepG2 (lane 3) and HCT116 (lane 4) using SLC40A1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • SLC40A1 Antibody Product Image
    1/16
  • Experimental Validation Results for SLC40A1 Antibody
  • SLC40A1 Antibody Product Image
  • Experimental Validation Results for SLC40A1 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 1) and K562 (lane 2) and HepG2 (lane 3) and HCT116 (lane 4) using SLC40A1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for SLC40A1 Antibody
  • SLC40A1 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 1) and K562 (lane 2) and HepG2 (lane 3) and HCT116 (lane 4) using SLC40A1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • SLC40A1 Antibody Product Image WB