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xCT Antibody (YA006)

(Synonyms: CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT)
13 Cited Publications
Customer Review

Based on 13 publication(s) in Google Scholar

xCT Antibody (YA006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to xCT.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide. Or Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • xCT Antibody (YA006) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) xCT Antibody (YA006)

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13 Publications Citing Use of MCE xCT Antibody (YA006) (7)
WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

xCT Antibody (YA006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to xCT.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human xCT.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102762

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide. Or Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for xCT Antibody (YA006)
    Western blot analysis of extracts from K562(lane 2(20μg), HepG2 (lane 3(20μg) and Hela(lane 4(20μg) using xCT (HY-P80935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    xCT is a Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress. The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine. In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis. Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis. In vitro can also transport L-aspartate. May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons. Controls the production of pheomelanin pigment directly[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Cell projection, microvillus membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is expressed in the term placenta and term primary trophoblast cells (PubMed:34120018) . It is mainly expressed in the brain, but also in the pancreas (PubMed:11417227) .

    Induction:By oxygen in a concentration-dependent manner (PubMed:11213471) . Up-regulated by S-nitroso-N-acetyl-D-penicillamine (PubMed:11133847)

  • Subunit

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc; this interaction mediates cell membrane localization

  • SwissProt ID

    Q9UPY5

  • Gene ID
    23657 [NCBI]
  • Synonyms

    CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT

  • Research Field

    Signal Transduction

[1]. Bridges CC, et al. Structure, function, and regulation of human cystine/glutamate transporter in retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2001 Jan;42(1):47-54. [Content Brief]

[2]. Bassi MT, et al. Identification and characterisation of human xCT that co-expresses, with 4F2 heavy chain, the amino acid transport activity system xc-. Pflugers Arch. 2001 May;442(2):286-96. [Content Brief]

[3]. Jiménez-Vidal M, et al. Thiol modification of cysteine 327 in the eighth transmembrane domain of the light subunit xCT of the heteromeric cystine/glutamate antiporter suggests close proximity to the substrate binding site/permeation pathway. J Biol Chem. 2004 Mar 19;279(12):11214-21. [Content Brief]

[4]. Gasol E, et al. Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility. J Biol Chem. 2004 Jul 23;279(30):31228-36. [Content Brief]

[5]. Parker JL, et al. Molecular basis for redox control by the human cystine/glutamate antiporter system xc(). Nat Commun. 2021 Dec 8;12(1):7147. [Content Brief]

[6]. Fiore A, et al. Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling. Mol Cell. 2022 Mar 3;82(5):920-932.e7. [Content Brief]

[7]. Yan R, et al. The structure of erastin-bound xCT-4F2hc complex reveals molecular mechanisms underlying erastin-induced ferroptosis. Cell Res. 2022 Jul;32(7):687-690. [Content Brief]

[8]. Lofthouse EM, et al. N-acetylcysteine, xCT and suppression of Maxi-chloride channel activity in human placenta. Placenta. 2021 Jul;110:46-55. [Content Brief]

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xCT Antibody (YA006) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg), HepG2 (lane 3(20μg) and Hela(lane 4(20μg) using xCT (HY-P80935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Feb 1:170:116049. [Abstract]

    Customer Validation
     The expressions of ferroptosis-associated proteins (i.e., ACSL4, xCT, and GPX4) in HPMVECs were determined by western blotting.

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Feb 1:170:116049. [Abstract]

    Customer Validation
     The expressions of ferroptosis-associated proteins (i.e., ACSL4, xCT, and GPX4) in HPMVECs were determined by western blotting.

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 1:35:102498. [Abstract]

    Customer Validation
     Western blot analyses were performed to evaluate proteins involved in ferroptosis signaling pathways in bone marrow-derived dendritic cells (BMDCs). Protein expressionan alysis further supported these findings, showing that LipNaB@Sor robustly inhibited the SLC7A11-GPX4 pathway, disrupted the P62-NRF2-KEAP1 pathway, and decreased FTH1 expression.

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 1:35:102498. [Abstract]

    Customer Validation
     Protein expression levels of GPX4, SLC7A11, and γH2AX across different treatment groups were assessed.

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2025 Jun 4:16:1567942. [Abstract]

    Customer Validation
     Western blotting was used to detect the expression of ferroptosis-related proteins in lung cancer cells after 24 h of PA with or without DFO (20 μM).

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156095. [Abstract]

    Customer Validation
     WB analysis showing the relative protein expression of GPX4, SLC7A11 (xCT), and ACSL4 in OGD/R-induced IEC-6 cells (n = 3-5).

  • 1/16

    xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 Dec;38(12):5583-5597. [Abstract]

    Customer Validation
     Representative western blotting and quantitative analysis of TFR1, SLC7A11 (xCT), and COX‐2 protein levels in U251 cells with the treatment indicated.

  • xCT Antibody (YA006) Product Image
    1/16
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • Experimental Validation Results for xCT Antibody (YA006)
  • xCT Antibody (YA006) Product Image
  • Experimental Validation Results for xCT Antibody (YA006)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg), HepG2 (lane 3(20μg) and Hela(lane 4(20μg) using xCT (HY-P80935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for xCT Antibody (YA006)

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Feb 1:170:116049. [Abstract]

    The expressions of ferroptosis-associated proteins (i.e., ACSL4, xCT, and GPX4) in HPMVECs were determined by western blotting.

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2026 Feb 1:170:116049. [Abstract]

    The expressions of ferroptosis-associated proteins (i.e., ACSL4, xCT, and GPX4) in HPMVECs were determined by western blotting.

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 1:35:102498. [Abstract]

    Western blot analyses were performed to evaluate proteins involved in ferroptosis signaling pathways in bone marrow-derived dendritic cells (BMDCs). Protein expressionan alysis further supported these findings, showing that LipNaB@Sor robustly inhibited the SLC7A11-GPX4 pathway, disrupted the P62-NRF2-KEAP1 pathway, and decreased FTH1 expression.

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 1:35:102498. [Abstract]

    Protein expression levels of GPX4, SLC7A11, and γH2AX across different treatment groups were assessed.

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2025 Jun 4:16:1567942. [Abstract]

    Western blotting was used to detect the expression of ferroptosis-related proteins in lung cancer cells after 24 h of PA with or without DFO (20 μM).

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156095. [Abstract]

    WB analysis showing the relative protein expression of GPX4, SLC7A11 (xCT), and ACSL4 in OGD/R-induced IEC-6 cells (n = 3-5).

  • xCT Antibody (YA006) purchased from MedChemExpress. Usage Cited in: Phytother Res. 2024 Dec;38(12):5583-5597. [Abstract]

    Representative western blotting and quantitative analysis of TFR1, SLC7A11 (xCT), and COX‐2 protein levels in U251 cells with the treatment indicated.

  • xCT Antibody (YA006) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg), HepG2 (lane 3(20μg) and Hela(lane 4(20μg) using xCT (HY-P80935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • xCT Antibody (YA006) Product Image WB