xCT Antibody (YA006)

Cat. No.: HY-P80935: Data Sheet COA
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xCT Antibody (YA006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to xCT.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
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xCT Antibody (YA006) Featured Recommendations:

12 Publications Citing Use of MCE xCT Antibody (YA006)

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Mater Today Bio. 2025 Nov 1:35:102498. [Abstract]; Western blot analyses were performed to evaluate proteins involved in ferroptosis signaling pathways in bone marrow-derived dendritic cells (BMDCs). Protein expressionan alysis further supported these findings, showing that LipNaB@Sor robustly inhibited the SLC7A11-GPX4 pathway, disrupted the P62-NRF2-KEAP1 pathway, and decreased FTH1 expression.

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Mater Today Bio. 2025 Nov 1:35:102498. [Abstract]; Protein expression levels of GPX4, SLC7A11, and γH2AX across different treatment groups were assessed.

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Front Pharmacol. 2025 Jun 4:16:1567942. [Abstract]; Western blotting was used to detect the expression of ferroptosis-related proteins in lung cancer cells after 24 h of PA with or without DFO (20 μM).

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Int Immunopharmacol. 2025 Dec 19:170:116049. [Abstract]; The expressions of ferroptosis-associated proteins (i.e., ACSL4, xCT, and GPX4) in HPMVECs were determined by western blotting.

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Int Immunopharmacol. 2025 Dec 19:170:116049. [Abstract]; The expressions of ferroptosis-associated proteins (i.e., ACSL4, xCT, and GPX4) in HPMVECs were determined by western blotting.

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Phytomedicine. 2024 Dec:135:156095. [Abstract]; WB analysis showing the relative protein expression of GPX4, SLC7A11 (xCT), and ACSL4 in OGD/R-induced IEC-6 cells (n = 3-5).

: xCT Antibody (YA006) purchased from MCE. Usage Cited in: Phytother Res. 2024 Dec;38(12):5583-5597. [Abstract]; Representative western blotting and quantitative analysis of TFR1, SLC7A11 (xCT), and COX‐2 protein levels in U251 cells with the treatment indicated.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

xCT Antibody (YA006) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to xCT.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9UPY5

Gene ID

23657 [NCBI]

Immunogen

Synthetic peptide corresponding to Human xCT.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide. Or Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20μg), HepG2 (lane 3(20μg) and Hela(lane 4(20μg) using xCT (HY-P80935) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:11133847, PubMed:11417227, PubMed:14722095, PubMed:15151999, PubMed:34880232, PubMed:35245456, PubMed:35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1:1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed:11133847, PubMed:11417227). In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed:35245456). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed:34120018). In vitro can also transport L-aspartate (PubMed:11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Cell projection, microvillus membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:It is expressed in the term placenta and term primary trophoblast cells (PubMed:34120018) . It is mainly expressed in the brain, but also in the pancreas (PubMed:11417227) .

Induction:By oxygen in a concentration-dependent manner (PubMed:11213471) . Up-regulated by S-nitroso-N-acetyl-D-penicillamine (PubMed:11133847)

Subunit：Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc; this interaction mediates cell membrane localization

RRID

AB_3102762

Database

Entrez Gene: 23657 Human ; 26570 Mouse ;

SwissProt: Q9UPY5 Human ; Q9WTR6 Mouse ;

OMIM: 607933 Human

Research Field

Signal Transduction

Synonyms

CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

xCT Antibody (YA006) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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xCT Antibody (YA006)

xCT Antibody (YA006) Featured Recommendations:

12 Publications Citing Use of MCE xCT Antibody (YA006)

Product Detail

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Background

Documentation

xCT Antibody (YA006) Related Classifications

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