ECT2 Antibody (YA7166)

Cat. No.: HY-P87479: Data Sheet User Guide for Antibodies
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ECT2 Antibody (YA7166) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to ECT2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ECT2 Antibody (YA7166) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to ECT2.

Host

Mouse

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 100kDa;

Species Reactivity

Human

SwissProt ID

Q9H8V3

Immunogen

KLH conjugated synthetic peptide derived from human ECT2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000

Sensitivity	Endogenous	Purity	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1, k

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death

Subcellular Localization：Nucleus,Cytoplasm,Cytoplasm, cytoskeleton, spindle,Cleavage furrow,Midbody,Cell junction,Cell junction, tight junction,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Expression：
Tissue_Specificity: Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma
Induction: Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR)

Isoforms & Post-Translational Modification：Q9H8V3 has four isomers: Q9H8V3-1: 103505 Da (predicted); Q9H8V3-2: 99921 Da (predicted); Q9H8V3-3: 108529 Da (predicted); Q9H8V3-4: 100051 Da (predicted).
Phosphorylated by PLK1 in vitro

Subunit：Homodimer (PubMed:14645260, PubMed:15545273, PubMed:16170345)

Documentation

User Guide for Antibodies (1077 KB)