ECT2 Antibody (YA7166)
(Synonyms: Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2)ECT2 Antibody (YA7166) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to ECT2.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB
-
Reactivity :
Human
-
Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:500-1000 |
Product Details
ECT2 Antibody (YA7166) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to ECT2.
-
Host Mouse
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman
-
Calculated Molecular Weight Predicted band size: 100kDa;
KLH conjugated synthetic peptide derived from human ECT2
Endogenous
affinity purified by Protein G
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death
-
Subcellular Localization
Nucleus,Cytoplasm,Cytoplasm, cytoskeleton, spindle,Cleavage furrow,Midbody,Cell junction,Cell junction, tight junction,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
-
Expression
Tissue_Specificity: Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma
Induction: Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR) -
Isoforms & Post-Translational Modification
Q9H8V3 has four isomers: Q9H8V3-1: 103505 Da (predicted); Q9H8V3-2: 99921 Da (predicted); Q9H8V3-3: 108529 Da (predicted); Q9H8V3-4: 100051 Da (predicted).
Phosphorylated by PLK1 in vitro -
Subunit
Homodimer (PubMed:14645260, PubMed:15545273, PubMed:16170345)
-
SwissProt ID
-
Synonyms
Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2
Documentation
Powered by Bioz
