CD8 alpha Antibody (YA526)(PBS only)
(Synonyms: CD8A; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a)CD8 alpha Antibody (YA526) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD8 alpha.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Hamster
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
CD8 alpha Antibody (YA526) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD8 alpha.
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Host Rabbit
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Species ReactivityHuman, Hamster
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Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26 kDa
Synthetic peptide corresponding to Human CD8.The exact sequence is proprietary to MCE.
affinity purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from K562 using CD8α antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Background
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Function
CD8a (CD8α) is a key component of the CD8 co-receptor and is primarily expressed on cytotoxic CD8+ T cells. By enhancing TCR-MHC I signaling, it promotes T-cell activation, IFN-γ production, and granzyme/perforin-mediated cell killing; it is one of the most commonly used T-cell markers in tumor immunology, infection immunology, vaccine research, and CAR-T cell research.
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Secreted
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Expression
Tissue_specificity:CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and dendritic cells expresses CD8A homodimers. Expressed at the cell surface of plasmacytoid dendritic cells upon herpes simplex virus-1 stimulation -
Isoforms & Post-Translational Modification
CD8a has 3 isomers: P01732-1: 25729 Da (predicted); P01732-2: 21585 Da (predicted); P01732-3: 30214 Da (predicted).
Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts;O-glycosylated;Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation -
Subunit
Forms disulfide-linked heterodimers with CD8B at the cell surface. Also forms homodimers in several cell types including NK-cells or peripheral blood T-lymphocytes.
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SwissProt ID
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Synonyms
CD8A; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD antigen CD8a
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Research Field
Immunology
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