Phospho-CD18 (Ser745) Antibody (YA220)

Cat. No.: HY-P80795: Ficha de datos COA
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Phospho-CD18 (Ser745) Antibody (YA220) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-CD18 (Ser745).

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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Phospho-CD18 (Ser745) Antibody (YA220) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Phospho-CD18 (Ser745) Antibody (YA220) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-CD18 (Ser745).

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular

Predicted band size: 85 kDa;

Observed band size: 85 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P05107

Gene ID

3689 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser745 of Human CD18.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Pureza	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using Phospho-CD18 (Ser745) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992, PubMed:28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Membrane raft; Single-pass type I membrane protein

Expression：
Tissue_specificity:Leukocytes (PubMed:23775590) . Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980)

Subunit：Heterodimer of an alpha and a beta subunit (PubMed:20033057). The ITGB2 beta subunit associates with the ITGAL, ITGAM, ITGAX or ITGAD alpha subunits.

RRID

AB_3102976

Database

Entrez Gene: 3689 Human ; 16414 Mouse ; 309684 Rat

SwissProt: P05107 Human ; P11835 Mouse ;

OMIM: 116920 Human

Research Field

Tags & Cell Markers

Synonyms

ITGB2; CD18; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150; 95 subunit beta; Complement receptor C3 subunit beta; CD antigen CD18

Documentación

Select Batch:

Ficha de datos (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-CD18 (Ser745) Antibody (YA220) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-CD18 (Ser745) Antibody (YA220)

Phospho-CD18 (Ser745) Antibody (YA220) Recomendaciones destacadas:

Product Detail

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Background

Descripciòn

Phospho-CD18 (Ser745) Antibody (YA220) Related Classifications

Productos vistos recientemente:

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