MRAS Antibody (YA3080)
(Synonyms: NS11; M-RAs; RRAS3; R-RAS3)MRAS Antibody (YA3080) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MRAS.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 |
Product Details
MRAS Antibody (YA3080) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MRAS.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 24 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 24 kDa
Entrez Gene: 22808 Human ; 17532 Mouse ;
SwissProt: O14807 Human ; O08989 Mouse ; P97538 Rat
OMIM: 618499 Human
A synthetic peptide of human Mras
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MRAS is a Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation. The formation of the SMP complex only occurs when MRAS is GTP-bound. MRAS has low intrinsic GTPase activity and may require additional factors for activation. The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities[1][2][3][4][5][6][7].
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Subcellular Localization
Cell membrane; Lipid-anchor; Cytoplasmic side
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Expression
Tissue_specificity:Expression highly restricted to the brain and heart
Induction:By IL9/interleukin-9, but not by IL2/interleukin-2 or IL4/interleukin-4 -
Isoforms & Post-Translational Modification
O14807 has 2 isomers: O14807-1: 23846 Da (predicted); O14807-2: 15542 Da (predicted).
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Subunit
Component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (either PPP1CA, PPP1CB or PPP1CC) (PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670, PubMed:39809765). Interacts (active GTP-bound form) with both SHOC2 and PP1c (all isoforms) to form a tertiary complex; SHOC2 and PP1c preferably bind M-Ras/MRAS, but they also bind K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670, PubMed:39809765). Interacts with RGL3 (By similarity). Interacts (active GTP-bound form preferentially) with RGS14 (By similarity)
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SwissProt ID
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Synonyms
NS11; M-RAs; RRAS3; R-RAS3
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Research Field
Signal Transduction
Documentation
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