Vinculin Antibody (YA5897)
(Synonyms: VCL; Vinculin; Metavinculin)
Based on 1 Customer Validation
Vinculin Antibody (YA5897) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vinculin.
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Host:
Rabbit
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Isotype:
IgG/Kappa
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:4000-1:20000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
Vinculin Antibody (YA5897) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vinculin.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 124 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 124 kDa
Protein A
Non-conjugated
Unmodified
IgG/Kappa
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Vinculin is an Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion
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Subcellular Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell junction, adherens junction; Cell junction, focal adhesion; Cytoplasm, cytoskeleton; Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side; Cell projection, podosome
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Expression
Tissue_specificity:Metavinculin is a muscle-specific protein. -
Isoforms & Post-Translational Modification
P18206 has 3 isomers: P18206-1: 123799 Da (predicted); P18206-2: 116722 Da (predicted); P18206-3: 24904 Da (predicted).
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity);Acetylated; mainly by myristic acid but also by a small amount of palmitic acid -
Subunit
Exhibits self-association properties. Part of a complex composed of THSD1, PTK2/FAK1, TLN1 and VCL (PubMed:29069646).
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SwissProt ID
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Synonyms
VCL; Vinculin; Metavinculin
Documentation
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