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Pyruvate Dehydrogenase E2 Antibody (YA113)

(Synonyms: DLTA, DLAT, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PBC, PDC-E2, PDCE2)
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Based on 1 Customer Validation

Pyruvate Dehydrogenase E2 Antibody (YA113) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA113)
  • Pyruvate Dehydrogenase E2 Antibody (YA113) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:200

Product Details

Description

Pyruvate Dehydrogenase E2 Antibody (YA113) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 69 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Pyruvate Dehydrogenase E2.AA range:574-624.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA113)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Pyruvate Dehydrogenase E2 Antibody (HY-P80292) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Pyruvate Dehydrogenase E2 is an As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety (Probable). The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable)

  • Subcellular Localization

    Mitochondrion matrix

  • Subunit

    Interacts with PDK2 and PDK3 (PubMed:15861126, PubMed:17532006, PubMed:17683942, PubMed:18387944). Interacts with SIRT4 (PubMed:25525879). Interacts with PDHB (PubMed:20160912)

  • SwissProt ID

    P10515

  • Gene ID
    1737 [NCBI]
  • Synonyms

    DLTA, DLAT, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PBC, PDC-E2, PDCE2

  • Research Field

    Signal Transduction

Pyruvate Dehydrogenase E2 Antibody (YA113) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Pyruvate Dehydrogenase E2 Antibody (HY-P80292) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Pyruvate Dehydrogenase E2 Antibody (YA113) Product Image
    1/16
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA113)
  • Pyruvate Dehydrogenase E2 Antibody (YA113) Product Image
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA113)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Pyruvate Dehydrogenase E2 Antibody (HY-P80292) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA113)
  • Pyruvate Dehydrogenase E2 Antibody (YA113) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using Pyruvate Dehydrogenase E2 Antibody (HY-P80292) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Pyruvate Dehydrogenase E2 Antibody (YA113) Product Image WB