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Fbx32 Antibody (YA919)

(Synonyms: Fbx32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein.)
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Based on 1 publication(s) in Google Scholar

Fbx32 Antibody (YA919) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fbx32.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB; ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Fbx32 Antibody (YA919) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:2000 1:50-200

Product Details

Description

Fbx32 Antibody (YA919) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fbx32.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 41 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Species Reactivity Database
Immunogen

Recombinant protein within Human Fbx32 aa 1-160 / 355.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103197

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Fbx32 Antibody (YA919)
    Western blot analysis of extracts from A431(lane 2, 20μg), C2C12(lane 3, 20μg), K562(lane 4, 20μg) and HT-29(lane 5, 20μg) using Fbx32 Antibody (HY-P81082). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Fbx32 Antibody (YA919)
    Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fbx32 Antibody (YA919)
    Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Fbx32 is a Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Specifically expressed in cardiac and skeletal muscle

  • Isoforms & Post-Translational Modification

    Q969P5 has 2 isomers: Q969P5-1: 41637 Da (predicted); Q969P5-2: 30804 Da (predicted).

  • Subunit

    Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32

  • SwissProt ID

    Q969P5

  • Gene ID
    114907 [NCBI]
  • Synonyms

    Fbx32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein.

Fbx32 Antibody (YA919) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 2, 20μg), C2C12(lane 3, 20μg), K562(lane 4, 20μg) and HT-29(lane 5, 20μg) using Fbx32 Antibody (HY-P81082). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fbx32 Antibody (YA919) Product Image
    1/16
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Fbx32 Antibody (YA919) Product Image
  • Experimental Validation Results for Fbx32 Antibody (YA919)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 2, 20μg), C2C12(lane 3, 20μg), K562(lane 4, 20μg) and HT-29(lane 5, 20μg) using Fbx32 Antibody (HY-P81082). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Fbx32 Antibody (YA919)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fbx32 Antibody (YA919)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Experimental Validation Results for Fbx32 Antibody (YA919)
  • Fbx32 Antibody (YA919) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431(lane 2, 20μg), C2C12(lane 3, 20μg), K562(lane 4, 20μg) and HT-29(lane 5, 20μg) using Fbx32 Antibody (HY-P81082). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10000) was used for 1 hour at room temperature.

  • Fbx32 Antibody (YA919) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fbx32 Antibody (YA919) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling Fbx32 with Fbx32 Antibody (YA919) (HY-P81082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Fbx32 Antibody (YA919) ((HY-P81082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Fbx32 Antibody (YA919) Product Image WB
  • Fbx32 Antibody (YA919) Product Image ICC
  • Fbx32 Antibody (YA919) Product Image ICC