GSK3 beta Antibody (YA397)

Cat. No.: HY-P80497: FAQs
Data Sheet SDS COA User Guide for Antibodies

GSK3 beta Antibody (YA397) is a non-conjugated and Rabbit origined monoclonal antibody about 47 kDa, targeting to GSK3 beta. It can be used for WB,ICC/IF,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

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10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
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Description	GSK3 beta Antibody (YA397) is a non-conjugated and Rabbit origined monoclonal antibody about 47 kDa, targeting to GSK3 beta. It can be used for WB,ICC/IF,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.
Tag	Free
Molecular Weight	Predicted band size: 47 kDa
Purity	Protein A affinity purified.
Subcellular Location	Cytoplasm, Nucleus, Cell membrane.
Conjugation	Non-conjugated
Research Field	Neuroscience
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Host	Rabbit
Reactivity	Human, Mouse, Rat
SwissProt ID	SwissProt: P49841 Human;SwissProt: Q9WV60 Mouse;SwissProt: P18266 Rat
Dilution Ratio	WB: 1:500-1:2,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 Western blot analysis of extracts from K562(lane 2(20μg) ), Jurkat(lane 3(20μg) ) and A549(lane 4(20μg) ) using Phospho-GSK3 beta(Ser 9) (HY-P80462) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80462, 1/1000) and Loading control antibody (GSK-3 beta, HY-P80497, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature. Western blot analysis of extracts from Jurkat (lane 2(20μg) or (lane 3(40μg)), using Phospho-GSK3 (Tyr216Tyr279) (HY-P80819) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80819, 1/1000) and Loading control antibody ( GSK-3 beta, HY-P80497, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application	WB, ICC/IF, IHC-P, FC
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Immunogen	Synthetic peptide corresponding to Human GSK3 beta.AA range:360-400.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (206 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:: GSK3 beta Antibody (YA397)
Cat. No.:: HY-P80497
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Western blot analysis of extracts from K562(lane 2(20μg) ), Jurkat(lane 3(20μg) ) and A549(lane 4(20μg) ) using Phospho-GSK3 beta(Ser 9) (HY-P80462) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80462, 1/1000) and Loading control antibody (GSK-3 beta, HY-P80497, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from Jurkat (lane 2(20μg) or (lane 3(40μg)), using Phospho-GSK3 (Tyr216Tyr279) (HY-P80819) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80819, 1/1000) and Loading control antibody ( GSK-3 beta, HY-P80497, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

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GSK3 beta Antibody (YA397)

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