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HNF 4 alpha Antibody (YA3571)

(Synonyms: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha)
Customer Review

Based on 1 Customer Validation

HNF 4 alpha Antibody (YA3571) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HNF 4 alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • HNF 4 alpha Antibody (YA3571) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:100 1:200-1:3000 1:1000

Product Details

Description

HNF 4 alpha Antibody (YA3571) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HNF 4 alpha.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 53 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Immunogen

Synthetic peptide within Human HNF-4-alpha aa 21-70 / 474.

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718760

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using HNF 4 alpha Antibody (HY-P83775) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    Flow cytometric analysis of 1X10^6 HepG2 cells labeling HNF 4 alpha Antibody (YA3571) (HY-P83775 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    HNF 4 alpha transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells[1][2].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P41235 has 7 isomers: P41235-1: 52785 Da (predicted); P41235-2: 51619 Da (predicted); P41235-3: 46623 Da (predicted); P41235-4: 56139 Da (predicted); P41235-5: 50150 Da (predicted); P41235-6: 48984 Da (predicted); P41235-7: 43988 Da (predicted).
    Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA;Acetylation at Lys-458 lowers transcriptional activation by about two-fold

  • Subunit

    Homodimerization is required for HNF4-alpha to bind to its recognition site (PubMed:14982928). Interacts with CLOCK, BMAL1, CRY1, CRY2, PER1 and PER2 (PubMed:30530698). Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionally inactive (PubMed:28128295). Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers (PubMed:28128295)

  • SwissProt ID

    P41235

  • Gene ID
    3172 [NCBI]
  • Synonyms

    TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha

HNF 4 alpha Antibody (YA3571) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using HNF 4 alpha Antibody (HY-P83775) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling HNF 4 alpha Antibody (YA3571) (HY-P83775 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HNF 4 alpha Antibody (YA3571) Product Image
    1/16
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • HNF 4 alpha Antibody (YA3571) Product Image
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using HNF 4 alpha Antibody (HY-P83775) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling HNF 4 alpha Antibody (YA3571) (HY-P83775 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • Experimental Validation Results for HNF 4 alpha Antibody (YA3571)
  • HNF 4 alpha Antibody (YA3571) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using HNF 4 alpha Antibody (HY-P83775) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HNF 4 alpha Antibody (YA3571) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HNF 4 alpha Antibody (YA3571) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using HNF 4 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HNF 4 alpha Antibody (YA3571) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 HepG2 cells labeling HNF 4 alpha Antibody (YA3571) (HY-P83775 red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • HNF 4 alpha Antibody (YA3571) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HNF 4 alpha Antibody (YA3571) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HNF 4 alpha Antibody (HY-P83775) at 1/150 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HNF 4 alpha Antibody (YA3571) Product Image WB
  • HNF 4 alpha Antibody (YA3571) Product Image IHC-P
  • HNF 4 alpha Antibody (YA3571) Product Image IHC-P
  • HNF 4 alpha Antibody (YA3571) Product Image FC
  • HNF 4 alpha Antibody (YA3571) Product Image ICC
  • HNF 4 alpha Antibody (YA3571) Product Image ICC