RASSF1 Antibody (YA5156)

Cat. No.: HY-P85464: Data Sheet User Guide for Antibodies
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RASSF1 Antibody (YA5156) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to RASSF1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RASSF1 Antibody (YA5156) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to RASSF1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 39 kDa;

Species Reactivity

Human

SwissProt ID

Q9NS23

Gene ID

11186 [NCBI]

Immunogen

Purified recombinant human RASSF1 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Nucleus; Nucleus

Expression：
Tissue_specificity:Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells

Isoforms & Post-Translational Modification：Q9NS23 has 8 isomers: Q9NS23-1: 39219 Da (predicted); Q9NS23-2: 38814 Da (predicted); Q9NS23-3: 21899 Da (predicted); Q9NS23-4: 31226 Da (predicted); Q9NS23-5: 39265 Da (predicted); Q9NS23-6: 9937 Da (predicted); Q9NS23-7: 16120 Da (predicted); Q9NS23-9: 8656 Da (predicted).

Subunit：Interacts with MAP1S (PubMed:15205320, PubMed:15753381). Interacts with XPA (PubMed:10888881). Binds to the N-terminal of CDC20 during prometaphase (PubMed:14743218).

Synonyms

RASSF1; RDA32; Ras association domain-containing protein 1

Documentation

User Guide for Antibodies (1077 KB)