alpha A Crystallin Antibody (YA2495)
(Synonyms: Acry 1; CRYA1; CRYAA; HspB4)alpha A Crystallin Antibody (YA2495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha A Crystallin.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 |
Product Details
alpha A Crystallin Antibody (YA2495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha A Crystallin.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 17 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 20 kDa
Entrez Gene: 102724652 Human ; 12954 Mouse ; 24273 Rat
SwissProt: P02489 Human ; P24622 Mouse ; P24623 Rat
OMIM: 604219 Human
A synthesized peptide derived from human CRYAA aa6-95.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
alpha A Crystallin contributes to the transparency and refractive index of the lens. In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions. Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Nucleus
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Expression
Tissue_specificity:Expressed in the eye lens (at protein level) -
Subunit
Heteropolymer composed of three CRYAA and one CRYAB subunits (PubMed:20836128). Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens (PubMed:22890888). Can also form homodimers and homotetramers (dimers of dimers) which serve as the building blocks of homooligomers (PubMed:17909943, PubMed:23255486, PubMed:31792453). Within homooligomers, the zinc-binding motif is created from residues of 3 different molecules. His-100 and Glu-102 from one molecule are ligands of the zinc ion, and His-107 and His-154 residues from additional molecules complete the site with tetrahedral coordination geometry (By similarity). Part of a complex required for lens intermediate filament formation composed of BFSP1, BFSP2 and CRYAA (PubMed:28935373)
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SwissProt ID
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Synonyms
Acry 1; CRYA1; CRYAA; HspB4
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Research Field
Signal Transduction
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