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Phospho-Smad5 (Ser463/465) Antibody (YA2838)

(Synonyms: DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad 5; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD mothers against decapentaplegic homolog 5; MAD; mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD mothers against DPP homolog 5; Smad5; Smad5; SMAD5_HUMAN.)
Customer Review

Based on 1 Customer Validation

Phospho-Smad5 (Ser463/465) Antibody (YA2838) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Smad5 (Ser463/465).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Phospho-Smad5 (Ser463/465) Antibody (YA2838) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Smad5 (Ser463/465).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Phospho-Smad5 (S463/465) IS-pS-V-pS.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3105209

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Smad5 Antibody (HY-P83093) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    SMAD5 is a receptor activated Smad (R-Smad), also known as dwarfin C, JV5-1 and MAD5, which belong to the dwarfin/SMAD family. The SMAD5 protein consists of an N-terminal domain responsible for DNA binding, a C-terminal domain primarily used for protein-protein interactions, and a junction region containing moths necessary for ubiquitination degradation. SMAD5 is activated by an activated type I TGF-β receptor, and phosphorylated SMAD5 binds to SMAD4 (common SMAD) to form heterotrimer complexes that enter the nucleus as transcription factors. At the same time, SMAD4 is also bound by inhibitory SMAD (SMAD6 and SMAD7), making R-Smad compete for receptors or interact with Smad4, and target degradation and negative regulation of TGF-β signaling. SMAD5 mainly responds to BMP signaling pathways. During embryonic development, SMAD5 is widely expressed in mice from gastrum formation. During this period, the loss of SMAD5 leads to mutations in the embryo, or defects in the yolk sac outside the embryo, and death in the second trimester. Furthermore, the BMP/SMAD5 pathway can regulate osteogenesis by up-regulating transcription of the transcription factor Runx2, or by targeting multiple cis-acting promoter elements in osteogenic specific genes, including osteocalcin and alkaline phosphatase (ALP) . Alps bind to Smad interacting protein 1 (SIP1) and inhibit Smad5-induced SIP1 activation. A significant expansion of primitive multipotent progenitor cells showing normal terminal maturation was reported in embryonic stem cells from Smad5-deficient mice, suggesting that conditional activation or deactivation of Smad5-specific signaling pathways may contribute to hematopoietic and osteogenic tissue engineering[1].

  • Subcellular Localization

    Cytoplasm; Nucleus; Mitochondrion

  • Expression


    Tissue_specificity:general expression

  • Subunit

    Homodimer (PubMed:33510867). Forms trimers with the co-SMAD SMAD4 (PubMed:9442019). Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3. Interacts with WWP1. Interacts with TMEM119 (By similarity). Interacts with ZNF8 (PubMed:12370310). Interacts with RANBP3L (PubMed:25755279). Interacts with HK1 (PubMed:28675158). Interacts with HGS; this interaction attenuates BMP signaling (PubMed:16516194)

  • SwissProt ID

    Q99717

  • Gene ID
    4090 [NCBI]
  • Synonyms

    DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad 5; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD mothers against decapentaplegic homolog 5; MAD; mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD mothers against DPP homolog 5; Smad5; Smad5; SMAD5_HUMAN.

  • Research Field

    Signal Transduction

References

[1]. Liu B, et al. Smad5: signaling roles in hematopoiesis and osteogenesis. Int J Biochem Cell Biol. 2004 May;36(5):766-70. [Content Brief]

Phospho-Smad5 (Ser463/465) Antibody (YA2838) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Smad5 Antibody (HY-P83093) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image
    1/16
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Smad5 Antibody (HY-P83093) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Experimental Validation Results for Phospho-Smad5 (Ser463/465) Antibody (YA2838)
  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Smad5 Antibody (HY-P83093) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Smad5 (Ser463/465) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image WB
  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image IHC-P
  • Phospho-Smad5 (Ser463/465) Antibody (YA2838) Product Image IHC-P