NEDD8 Activating Enzyme E1C Antibody (YA9707)
(Synonyms: DKFZp566J164, EC 6.3.2., hUba3, MGC22384, NEDD8 activating enzyme E1 catalytic subunit, NEDD8 activating enzyme E1C, Nedd8 activating enzyme hUba3, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, uba3)NEDD8 Activating Enzyme E1C Antibody (YA9707) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to NEDD8 Activating Enzyme E1C.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:200 | 1:1000 |
Product Details
NEDD8 Activating Enzyme E1C Antibody (YA9707) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to NEDD8 Activating Enzyme E1C.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 52 kDa;
Recombinant protein of Human UBA3 (aa 220-424).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NEDD8 Activating Enzyme E1C is a Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression
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Expression
Tissue_Specificity: Ubiquitously expressed. -
Isoforms & Post-Translational Modification
NEDD8 Activating Enzyme E1C has 2 isoforms, Q8TBC4-1: amino acid length is 463, molecular weight is 51852 Da (predicted); Q8TBC4-2: amino acid length is 449, molecular weight is 50072 Da (predicted).
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Subunit
Heterodimer of UBA3 and NAE1.
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SwissProt ID
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Synonyms
DKFZp566J164, EC 6.3.2., hUba3, MGC22384, NEDD8 activating enzyme E1 catalytic subunit, NEDD8 activating enzyme E1C, Nedd8 activating enzyme hUba3, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, uba3
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