DDR1 Antibody (YA4811)
(Synonyms: DDR1; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain-containing receptor 1; Epithelial discoidin domain receptor 1; CD167 antigen-like family member A; Cell adhesion kinase; Discoidin receptor tyrosine kinase; HGK2; )DDR1 Antibody (YA4811) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to DDR1.
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Host:
Mouse
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Application:
WB, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:10000 |
Product Details
DDR1 Antibody (YA4811) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to DDR1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 101 kDa;
Purified recombinant fragment of DDR1 (aa602-681) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
DDR1 is a collagen-activated receptor tyrosine kinase that transduces collagen binding into signaling controlling adhesion, proliferation, differentiation, migration, and matrix homeostasis[1]. Mechanistically, DDR1 responds to triple-helical collagen through slow, sustained autophosphorylation, while N-glycosylation at Asn211 restrains ligand-independent phosphorylation[2]. DDR1 signaling connects extracellular matrix sensing to MAPK, PI3K/Akt, NF-κB, RhoA/ROCK/MAPK/ERK, and PI3K/AKT/mTOR pathways in inflammatory and fibrotic disease models[3][4][5]. In cancer models, collagen I-activated DDR1 promotes N-cadherin up-regulation in pancreatic cancer, migration and invasion in hepatocellular carcinoma cells, and chemoresistance signaling in breast cancer cells[6][7][8]. Compared with related isoforms, DDR1b, but not DDR1a, mediates collagen I-induced N-cadherin up-regulation through Tyr513, Shc1, and Pyk2 coupling in pancreatic cancer cells[6]. Compared with DDR2, DDR1 inhibition, but not DDR2 inhibition, reduced collagen I expression and improved macromolecular delivery in a 3D pancreatic ductal adenocarcinoma fibrosis model[5]. For experimental applications, imatinib, ponatinib, and DDR1-IN-1 inhibit DDR kinases, and DDR1-IN-1 shows structural selectivity and induces autophagy-associated necroptotic death in malignant peripheral nerve sheath tumor cells[9][10].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted; Cell membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level) . Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin -
Isoforms & Post-Translational Modification
Q08345 has 5 isomers: Q08345-1: 101128 Da (predicted); Q08345-2: 97174 Da (predicted); Q08345-4: 27130 Da (predicted); Q08345-5: 101796 Da (predicted); Q08345-6: 99064 Da (predicted).
Autophosphorylated in response to fibrillar collagen binding;Glycosylation of Asn-211, but apparently not of Asn-260 or Asn-394, prevents autophosphorylation from occurring in the absence of collagen -
Subunit
Homodimer. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2
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SwissProt ID
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Synonyms
DDR1; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain-containing receptor 1; Epithelial discoidin domain receptor 1; CD167 antigen-like family member A; Cell adhesion kinase; Discoidin receptor tyrosine kinase; HGK2;
Documentation
References
[1]. Kothiwale S, et al. Discoidin domain receptor 1 (DDR1) kinase as target for structure-based drug discovery. Drug Discov Today. 2015 Feb;20(2):255-61. [Content Brief]
[2]. Schmoldt A, et al. Digitoxin metabolism by rat liver microsomes. Biochem Pharmacol. 1975 Sep 1;24(17):1639-41. [Content Brief]
[3]. Dagamajalu S, et al. A network map of discoidin domain receptor 1(DDR1)-mediated signaling in pathological conditions. J Cell Commun Signal. 2023 Sep;17(3):1081-1088. [Content Brief]
[4]. El Azreq MA, et al. Discoidin domain receptor 1 promotes Th17 cell migration by activating the RhoA/ROCK/MAPK/ERK signaling pathway. Oncotarget. 2016 Jul 19;7(29):44975-44990. [Content Brief]
[5]. Ohira M, et al. Collagen Signaling via DDR1 Exacerbates Barriers to Macromolecular Drug Delivery in a 3D Model of Pancreatic Cancer Fibrosis. Small. 2025 Dec;21(50):e06926. [Content Brief]
[6]. Huang H, et al. Up-regulation of N-cadherin by Collagen I-activated Discoidin Domain Receptor 1 in Pancreatic Cancer Requires the Adaptor Molecule Shc1. J Biol Chem. 2016 Oct 28;291(44):23208-23223. [Content Brief]
[8]. Baltes F, et al. Targeting Discoidin Domain Receptor 1 (DDR1) Signaling and Its Crosstalk with β1-integrin Emerges as a Key Factor for Breast Cancer Chemosensitization upon Collagen Type 1 Binding. Int J Mol Sci. 2020 Jul 13;21(14):4956. [Content Brief]
[9]. Canning P, et al. Structural mechanisms determining inhibition of the collagen receptor DDR1 by selective and multi-targeted type II kinase inhibitors. J Mol Biol. 2014 Jun 26;426(13):2457-70. [Content Brief]
[10]. Lai GY, et al. Discoidin domain receptor inhibitor DDR1-IN-1 induces autophagy and necroptotic cell death in malignant peripheral nerve sheath tumor. Cell Death Discov. 2025 Mar 1;11(1):83. [Content Brief]
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