FADD Antibody (YA2793)

Cat. No.: HY-P83048: Data Sheet COA
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FADD Antibody (YA2793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADD.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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FADD Antibody (YA2793) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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References

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FADD Antibody (YA2793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADD.

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 23 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q61160

Gene ID

8772 [NCBI]

Immunogen

A synthetic peptide of mouse FADD

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from 3T3 (lane 1, 20 μg), 3T3+CA (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), and SH-SY5Y+CA (lane 4, 20 μg) using FADD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling

Subcellular Localization：Cytoplasm

Subunit：Can self-associate (By similarity). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (PubMed:34471287). Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95 or TNFRSF1A, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (By similarity). Interacts (via death domain) with FAS (via death domain) (By similarity). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (PubMed:15383280). Interacts with CFLAR, PEA15 and MBD4 (By similarity). When phosphorylated, part of a complex containing HIPK3 and FAS (By similarity). May interact with MAVS/IPS1 (By similarity). Interacts with MOCV v-CFLAR protein and PIDD1 (By similarity). Interacts with RIPK1 and TRADD (PubMed:28842570, PubMed:29440439, PubMed:30185824, PubMed:31519887). Interacts with stimulated TNFRSF10B (By similarity). Interacts with DDX24 (By similarity)

RRID

AB_3105164

Database

Entrez Gene: 8772 Human ; 14082 Mouse ;

SwissProt: Q13158 Human ; Q61160 Mouse ;

OMIM: 613759 Human

Research Field

Cell Biology

Synonyms

Mort1/FADD

각종 서류

Select Batch:

Data Sheet (258 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mcf7-cell.html [Content Brief]

FADD Antibody (YA2793) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FADD Antibody (YA2793)

FADD Antibody (YA2793) Featured Recommendations:

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FADD Antibody (YA2793) Related Classifications

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